目的观察间质胶原酶(MMP - 1)表达质粒对大鼠肝纤维化的影响。
Objective To observe tile effects of MMP-1 (Matrix Metalloproteinase-1) expressing plasmid on rat liver fibrosis.
逆转录多聚酶链反应检测间质胶原酶及金属蛋白酶组织抑制因子- 1基因表达水平。
The gene expression levels for interstitial collagenase and tissue inhibitor of metallo-proteinase-1 (TIMP-1) were measured by reverse transcription polymerase chain reaction.
无菌条件下取正常足月剖腹产的脐带近胎儿段，剔除动静脉，取出其中的间质组织，胶原酶消化法原代培养细胞进行培养和纯化获得贴壁细胞。
Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.
无菌条件下取正常足月剖腹产的脐带近胎儿段，剔除动静脉，取出其中的间质组织，胶原酶消化法原代培养细胞进行培养和纯化获得贴壁细胞。
Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.
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