建立了间接荧光免疫法检测纯化抗体的方法。
An indirect fluorescence immunoassay to detect antibody was developed.
采用间接免疫荧光标记方法观察PKC及锚蛋白的细胞内分布情况。
The cellular distribution of PKC and ankyrin was observed by indirect immunofluorescence.
采用间接免疫荧光法(IFA)检测外源基因的表达。
The expression of exogenous genes were analyzed by indirect immunofluorescence assay (IFA).
采用间接免疫荧光法(IFA)检测外源基因的表达。
The exogenous gene expression was analyzed by indirect immunofluorescence assay (IFA).
方法：采用间接免疫荧光标记，流式细胞术分析。
Methods Indirect immune fluorescent labeling and flow cytometry were employed.
间接免疫荧光、ELISA和免疫印迹检测证明，含SO.7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
方法：间接免疫荧光试验等。
方法应用MTT、CFU - L及间接免疫荧光法对18例急性白血病进行观察。
Methods MTT, CFU-L and indirect immune fluorescence were used in 18 patients with acute leukemias.
其敏感性比间接免疫荧光法（IFA）高数十倍；
方法：采用间接免疫荧光流式细胞术，分析K562细胞表面TRAIL分子的表达。
METHODS: The expression of TRAIL on K562 cells was detected by indirect immunofluorescence staining and flow cytometry.
用间接免疫荧光染色结合流式细胞技术检测细胞表面DR4和DR5分子表达；
The expression of cell surface-bound DR4 and DR5 were determined by indirect fluorescence staining and flow cytometry analysis.
目的建立亚马逊利什曼原虫无鞭毛体体外培养方法，并用间接免疫荧光法分析其抗原特异性。
Objective To establish a method for culture of Leishmania amazonensis amastigotes in vitro and identify the antigenic specificity of amastigotes with indirect immunofluorescent assay.
方法：本研究采用间接免疫荧光法测定T细胞亚群；
Method:The indirect immunofluorescent assay was used in the present study.
目的：观察超声成像及间接免疫荧光技术在骨髓基质细胞移植治疗缺血心肌病方面的评估价值。
AIM To observe the evaluation value of ultrasonic imaging and indirect immunofluorescence technique in the treatment of ischemic cardiomyopathy with marrow stroma cells MSCs transplantation.
上述处理后的细胞经间接免疫荧光染色标记后，用激光共聚焦显微镜观察其细胞膜egf受体的聚簇现象。
EGF receptors on the cell membrane were observed under a laser scanning confocal microscope after indirect immunofluorescence staining.
间接免疫荧光染色显示，小鼠早期胚胎细胞对角蛋白抗体呈阳性反应，对波形蛋白和结蛋白抗体呈阴性反应。
Indirect immunofluorescence detection showed that positive reaction of diffuse cytoplasmic fluorescence was seen with antibody to keratin but not with antibodies to vimentin and desmin.
方法应用间接免疫荧光(IIF)和ELISA方法检测了205例肾脏疾病患者血清中的ANCA及其靶抗原。
Methods ANCA fluorescent patterns and its target antigens in sera of 205 patients with renal diseases were determined by indirect immunofluorescence (IIF) and ELISA.
间接免疫荧光染色及流式细胞术鉴定多克隆抗体的特异性。
The specificity of the purified polyclonal antibody was identified by indirect fluorescence staining and FCM analysis.
方法采用间接免疫荧光法检测白血病患者T淋巴细胞亚群、NK细胞百分数，MTT法检测NK细胞杀伤活性。
Methods t lymphoid subgroups and the percentage of NK cells were detected by the use of indirect immunofluorescence method the function of NK cells was detected by MTT.
继用抗S-100蛋白单抗通过间接免疫荧光法及ABC法进行细胞鉴定。
The cells were identified with anti S100 protein antibody by indirect immunofluoresent methods and ABC methods.
间接免疫荧光法检测血清中抗核抗体(ANA)的荧光滴度。
The serum fluorescence titres of anti-nuclear antibodies (ANA) were tested by indirect immunofluorescence technique.
方法：间接免疫荧光标记法。
运用透射电镜、钙染色和革兰染色等手段观察其形态特征，采用单克隆抗体间接免疫荧光染色进行细菌鉴定。
The obtained cells were observed using transmission electron microscopy(TEM), detected with alizarin red staining and Gram staining, and identified by indirect immunofluorescence staining .
应用免疫金银染色技术（IGSS）和间接荧光抗体试验（IFAT）对日本血吸虫、华支睾吸虫和卫氏肺吸虫进行抗原定位的研究。
The antigen location of Schistosoma japonicum, Clonorchis sinensis and Paragonimus westermani were studied by using immunogold-silver staining(IGSS) and indirect fluorescent antibody test(IFAT).
用胰腺冰冻切片间接免疫荧光法成功地检测出血清中胰岛细胞抗体(ICA)。
Islet cell antibodies (ICA) were successfully detected with indirect immunofluorescence assay on frozen sections of human pancreas.
方法采用间接免疫荧光法分析102例初治all患者的免疫表型。
Method Immunophenotypes were examined using indirect immunofluorescence method in 102 denovo ALL.
方法：采用间接免疫荧光法、小鼠保护试验以及SDS -聚丙烯胺电泳法，分别测定分离株的血清类型、毒力和蛋白图谱。
Method: Use the method of indirect immunofluorescence, mice cross protection test and SDS -page to determine the serum type, the toxicity and the protein atlas of the isolated strains.
E7基因表达的检测采用RTPCR和间接免疫荧光实验。
The expression of E7 gene was examined by RT PCR and the indirect immunofluorescence.
间接免疫荧光检测和IMMS与细胞的结合实验证明，所构建的IMMS可有效地和靶细胞结合。
The results of indirect immunofluorescence and cell binding suggest that IMMS can effectively bind target cells while do not have nonspecific binding activity.
收集鼠肺和鼠血，用间接免疫荧光法检测鼠肺中抗原和鼠血中抗体。
Rat blood and lungs were collected for indirect immunofluorescent detection of related antigens and antibodies.
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