推导的氨基酸序列具有典型的抗体轻、重链可变区特征。
Two amino acid sequences deduced had typical features of Ig heavy and light chains' variable regions.
目的：获得抗人视网膜母细胞瘤单克隆抗体轻链可变区基因。
Objective: to acquire the variable region gene of light chain of monoclonal antibody against human retinoblastoma.
结果：克隆的基因序列符合小鼠轻、重链可变区基因的特征；
RESULTS: The VH and VL genes were homologous with the published gene sequences of mouse antibody variable region.
核苷酸序列分析表明所克隆基因分别为抗体轻、重链可变区基因。
The nucleotide sequence analyses indicated that the cloned genes coded the variable light and heavy chain domains from mouse antibodies respectively.
轻链可变区和恒定区均包含保守的半胱氨酸，形成链内和链间二硫键。
The cysteine residues involved in the formation of disulfide bonds are completely conserved among fish IgL.
目的获取系列鼠抗人CD分子单抗的轻链及重链可变区基因并进行克隆和测序。
Objective to obtain the VH and VL variable region genes of murine McAbs to human CD molecule.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
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