反转录-聚合酶链反应法检测CXCR4的表达，亦得到相似结果。
Similar results were obtained through detecting the expression of CXCR4 with reverse transcription-polymerase chain reaction.
方法 对62例无精症、少精症患者及20例正常男性采用多重聚合酶链反应法进行AZF区基因微缺失检测。
Methods PCR method was used to detect micro-deletion in AZF gene in 62 oligospermatism and azoospermatism patients and 20 normal male controls.
通过聚合链反应或ELISA酶标法测试，共有超过25例人类样本被证实为裂谷热阳性。
More than 25 human samples have proved positive for Rift Valley fever by PCR or ELISA testing.
通过聚合链反应或ELISA酶标法，已发现25例以上的人类样本为裂谷热阳性。
More than 25 human samples have been found positive for RVF by PCR or ELISA.
聚合酶链反应-微孔板杂交法检测输血传播病毒。
DNA detection of tt virus DNA by polymerase chain reaction -microplate hybridization.
目的探讨双重聚合酶链反应（DPCR）法检测痰及支气管肺泡灌洗液（BALF）中军团菌DNA在早期诊断军团菌肺炎的意义。
Objective To investigate the value of duplex polymerase chain reaction (DPCR) in early diagnosis of Legionella pneumonia by detecting Legionella DNA in sputum and bronchoalvelar lavage fluid(BALF).
采用单链聚合酶链反应(PCR)和测序法检测肿瘤组织中VHL基因的突变情况。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
方法利用聚合酶链反应-序列特异引物(PCR -ssp)法，对189例银屑病患者和273例健康人的HLA - DQA1和DQB1等位基因进行检测。
Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls.
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
酚-氯仿法从外周血中提取基因组dna，聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
目的评价聚合酶链反应(PCR)分子灯塔法检测临床标本中结核分枝杆菌(结核菌)的应用价值。
Objective To evaluate the value of PCR molecular beacon assay. In detecting mycobacterium tuberculosis in clinical specimens.
方法采用聚合酶链反应扩增该家系患者和健康对照个体atp2c1基因的全部外显子，直接测序法进行DNA测序，100例无亲缘关系的正常人作为对照。
Method all exons of ATP2C1 gene were analyzed with polymerase chain reaction and DNA sequencing in all patients of this family and 100 unrelated population-match controls.
采用聚合酶链反应(PCR)方法扩增rho基因第1 ~ 5外显子和第1内含子基因片段，用直接dna测序法筛查rho基因突变。
Extron 1-5 of RHO gene was amplified by polymerase chain reaction (PCR), and the mutation of RHO gene was screened by direct DNA sequence measurement.
聚合酶链反应和病毒分离培养法对50例孕妇（怀51胎）进行评价的阳性预测值分别为92.0%和93.7%。
The positive predictive values (PPV) of the PCR and virus isolation assessments performed in all 50 pregnancies (51 gestational sacs) were 92% and 93.7%, respectively.
根据幕斯塔法的研究，科学家可以使用一种叫做聚合酶链反应(又称PCR)的程序来进行检测，这种程序使得出检测结果的时间缩短至5至12个小时。
Mustapha's research allows scientists to use a process, known as polymerase chain reaction (PCR), which can cut testing time to as little as five to 12 hours.
结果：32例阳性标本经过聚合酶链杂交结果与常规鉴定结果相符。结论：聚合酶链反应杂交梳法鉴定结核杆菌简便、快速、特异性强，有较高的临床应用推广价值。
Results The detective results of 32 positive samples with PCR hybridization combing is a quick, simple and highly specific method in the detection of TB, and may has some values in clinical diagnosis.
用逆转录-聚合酶链反应（RT-PCR）法进行检测。
The viral RNA was amplified by a reverse transcription polymerase chain reaction (RT-PCR).
目的建立敏感、特异、稳定的聚合酶链反应(pcr)法，以检测旋毛虫病。
Aim To establish a sensitive specific and stable polymerase chainreaction (PCR) for detecting Trichnilla spiralis infection.
方法：应用逆转录-聚合酶链反应(RT - pcr)法检测73例急性白血病患者及23例正常人的WT 1及LRP基因的表达。
Methods: Expressions of WT1 and LRP genes were measured in 73 patients with acute leukemia and 23 normal controls by RT-PCR method.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
方法采用逆转录聚合酶链反应(RT pcr)法。
采用多聚酶链反应-限制性片段长度多态性法(PCR - RFLP)分析MGP和ALAD基因的多态性。
The polymorphisms of MGP gene and ALAD gene were analyzed by the methods of PCR-RFLP.
聚合酶链反应-限制性片段长度多态性法；
Polymerase chain reaction restriction fragment length polymorphism (PCR RFLP);
方法采用微量稀释法检测21种抗菌药物的敏感性，运用聚合酶链反应（PCR）技术检测氨基糖苷类修饰酶基因。
METHODS The dilution test was performed to detect the susceptibility to 21 kinds of antimicrobial agents and genotypes of AMEs of 20 E. coli strains were analyzed by polymerase chain reaction(PCR).
方法采用微量稀释法检测21种抗菌药物的敏感性，运用聚合酶链反应（PCR）技术检测氨基糖苷类修饰酶基因。
METHODS The dilution test was performed to detect the susceptibility to 21 kinds of antimicrobial agents and genotypes of AMEs of 20 E. coli strains were analyzed by polymerase chain reaction(PCR).
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