经金属螯合层析纯化，获得了高纯度的表达蛋白。
Both proteins were purified with Nickel chelate affinity chromatography.
研究了应用金属螯合亲和层析法纯化重组类人胶原蛋白的条件。
Recombinant human-like collagen with a hexahistidine tail attacked to the carboxyl end could be easily purified by Metal Chelated Affinity Chromatography (MCAC).
后来发现它是一种与胰岛素样生长因子相关的锌指超家族中的金属螯合蛋白酶。
And then it was found that PAPP-A was a zinc-binding matrix metalloproteinase associated with insulin-like growth factor.
制备了以壳聚糖为载体的金属螯合亲和色谱分离纯化还原型谷胱甘肽(GSH)。
Immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione (GSH) using chitosan as substrate.
改变色谱条件可改变金属螯合配体和蛋白质间的作用力，提高金属螯合色谱的选择性。
The interaction between the metal chelate ligand and proteins and the selectivity of metal chelate chromatography can be changed through changing chromatographic conditions.
结论 利用毕赤酵母系统表达出高活性的、具有内在金属螯合位点的突变型人膜联蛋白V。
Conclusion Highly-active recombinant mutant human annexin V with endogenous metal-chelating sites can be expressed in Pichia Pastoris system.
考察了螯合型弱阳离子交换剂与金属离子的作用，研究了蛋白质在金属螯合色谱中的保留机理。
The interaction between chelating weak cation-exchanger and metal ions was examined. The retention mechanism of protein in metal chelating chromatography was explored.
现用于共振光散射法测定核酸的试剂主要有卟啉类化合物、金属螯合物和有机小分子染料三类。
Three kinds of agents are employed in resonance light scattering technique: porphyrin combinations, metal complexes and organic small molecular dyes.
本论文利用熔融石英毛细管为载体，制备了共价键合及金属螯合两种蛋白质水解酶固定化微反应器。
With fused silica capillary as support, immobilized enzyme microreactor has been prepared using covalent bonding or metal-ion chelating method.
采用补充金属螯合离子，能部分恢复亲和膜对溶菌酶的吸附量，是实现亲和膜重复使用的有效方法。
Adsorption amount of lysozyme can be partly restored by addition of metal ions on the reused affinity membranes.
有机金属化合物处理技术可采用的化合物有：有机金属螯合物、无色多孔材料、有机硅烷和聚磷酸铵。
The organic metal-containing compounds treatment technology can employ the compounds of chelate, colorless porous materials, organic silane.
采用高分子有机螯合剂与废水中的多种重金属离子发生螯合反应，生成稳定且不溶于水的金属螯合物来去除废水中的重金属离子。
Using organic chelating agent to response with several kinds of heavy metal ion, then the heavy metal ion will be reduced by forming the product with high stability and hardly dissolved by water.
在洗衣粉中掺入螯合剂，例如次氮基三乙酸酯(NTA)或者是乙二胺四乙酸酯(EDTA)，这些螯合剂易于形成水溶性的金属螯合物。
A chelating agent which forms an easily water-soluble metal chelated compound such as nitrilotriacetate (NTA) or ethylene diamine tetraacetate (EDTA) is incorporated in a detergent composition.
试验结果表明，采用金属螯合色谱可较好地分离提取免疫球蛋白，其纯度可达89%。同时还可分离得到副产品乳铁蛋白，但含量较低，仅为33%。
The results showed that the purity of Immunoglobulin isolated by MCIC was 89%, meanwhile Lactoferrin was obtained with relative low content (33%).
而要形成螯合物其中有一个因素非常重要，就是金属离子周围必须有足够的原子与它产生配位。
One of the important factors to form chelate is there should be enough atoms to coordinate with metal ion.
通过上述晶体结构中配位键长键角的分析，得到了一个行之有效的标准，判断羧基与中心金属离子是单齿配位还是螯合配位。
A new criterion to decide whether the carboxy group coordinating to the metallic ion in mono or bi dentate-fashion in an ambiguous situation, base on coordination bond distances and angles.
强相互作用吸附的半胱氨酸主要是以去质子的两性离子和阴离子形式存在于粘土矿物上，与过渡金属形成螯合的六元环结构。
The cysteine in the clay mineral existed in the forms of zwitterion and anion, the O and S in it chelated zinc cation to form configuration of hexatomic ring.
另一类是酰化花色素苷与辅助色素以分子间辅助着色方式而形成的复合花色素，这类花色素经常包含金属离子参与的螯合反应。
Another type is that the acylated anthocyanin complex with co-pigments, metals are cheated sometimes, and which abides by intermolecular co-pigmentation mechanism.
壳聚糖的乳化性能、絮凝能力和金属离子螯合能力对其应用，特别是对其在食品工业中的应用有重大影响。
The emulsifying characteristics, flocculating property and chelating ability of chitosan were important to its application, especially in the food industry.
根系分泌物可通过溶解、螯合、还原等作用活化土壤重金属，提高重金属的植物有效性，或固定和钝化重金属，降低重金属的移动性。
Root exudates could activate heavy metals in soil, and enhance their bio-availability by dissolving, chelating, deoxidizing, decrease migration of heavy metals by fixating and inactivating.
近年来，螯合诱导技术作为一种强化植物吸收重金属的修复技术备受关注。
As one kind of remediation technique to enhance accumulation of heavy metal in plants, Chelate-induced Phytoremediation has been emphasized more and more.
本发明涉及单克隆抗体及金属离子与螯合剂组成的螯合物，它既可用于治疗诊断，也可用于纯化微量金属等其它方面。
The invention relates to monoclonal antibodies and the chelate, composed of metal ions and chelating agent. It can be either used for curing and diagnosing or for purifying trace elements of metal.
以腐植酸为主要原料。通过缩合、 接枝共聚、 金属离子螯合和磺化等系列反应制得了高温抗盐防塌降滤失剂K CS-53。
The novel high temperature fluid loss additive named KCS-53 with functions of anti-caving and salt resistance is produced from humic acid by synthesis.
胺肟型螯合纤维的胺肟螯合基团可以与某些金属离子发生螯合作用，增强了吸附金属离子的定向性和选择性。
Chelation can be taken between amidoxime group in amidoxime chelate fiber and some metal ions. The adsorbent has better adsorbability.
所述活化剂可以是水溶性烷酰基-给体化合物或螯合过渡金属。
The activator can be a water-soluble alkanoyl-donor compound or a chelated transition metal.
最后对高分子絮凝剂的螯合机理进行了探讨，此外，通过红外光谱定性的分析了絮凝剂与重金属离子的螯合机理。
The synthesizing mechanism of macromolecule was discussed in the end. At the sme time IR was applied to study chelate mechanism.
最后对高分子絮凝剂的螯合机理进行了探讨，此外，通过红外光谱定性的分析了絮凝剂与重金属离子的螯合机理。
The synthesizing mechanism of macromolecule was discussed in the end. At the sme time IR was applied to study chelate mechanism.
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