当研究者将通过基因改良后的真菌孢子在实验室中释放到蚊子身上后，相对于野生型的真菌，这些改良后的真菌并没有更快的杀死蚊子。
When the researchers sprayed spores of the genetically modified fungus strains onto mosquitoes in the lab, the spores did not kill the insects faster than the normal fungus did.
目的探讨外源野生型PTEN抑癌基因对人高转移性黏液表皮样癌细胞系m 3sp2体外黏附、迁移和侵袭特性的影响。
Objective To study effects of the exogenous wild-type PTEN tumor suppressor gene on in vitro adhesion, migration and invasion of the highly metastatic mucoepidermoid carcinoma cell line M3SP2.
结果：与野生型腺病毒相比，重组腺病毒能显著地提高目的基因在四种不同肿瘤细胞中的表达。
Results Fiber-mutant adenovirus vector showed a notably enhanced gene expression in four different tumor cells compared with that of conventional ones.
结论野生型和突变型PRPF31基因真核表达载体的构建，为研究PRPF31基因突变引起视网膜色素变性的机制奠定了基础。
Conclusion Construction of the eukaryotic expression vectors of wild type and mutant PRPF31 genes is basic work for research on the mechanisms of retinitis pigmentosa caused by PRPF31 mutation.
结论转染野生型PTEN抑癌基因能降低人高转移性黏液表皮样癌细胞转移能力。
Conclusion Transfection of wild-type PTEN tumor suppressor gene might significantly reduce metastatic ability of human highly metastatic mucoepidermoid carcinoma cells.
目的构建人细胞色素P450 4f 2基因野生型、V81G和V433 M突变型表达载体，在大肠杆菌中表达并纯化出CYP4F2蛋白。
Objective to construct the expression vectors of human cytochrome P450 (CYP) 4f2 wild type, V81G and V433M Variants and express and purify CYP4F2 protein in e.
方法采用聚合酶链反应（PCR）扩增12例JAK2V617F点突变阴性PV患者的JAK2外显子12片段，经基因测序与野生型JAK2外显子12比对，了解是否存在JAK2外显子12突变。
Methods Polymerase chain reaction(PCR)was used for 12 JAK2V617F -negative PV patients to amply the region of JAK2 exon 12, direct gene sequencing was performed to detect mutations of JAK2 exon 12.
本文报道了在克隆了小鼠酪氨酸酶基因的基础上，在大肠杆菌表达体系中诱导表达野生型和变异型酪氨酸酶的结果。
Here we reported the cloning of the gene from murine tyrosinase, the expression of wild tyrosinase and variants in E. coli.
结果基因芯片技术监测能有效分辨野生型和突变型HBV。
Results This microarray could clearly differentiate the wide-type from mutated-type HBV with lamivudine-resistance mutations.
结论：外源野生型PTEN基因的转染能导致体外培养的高转移性黏液表皮样癌细胞变性、坏死或凋亡。
Conclusion: the exogenous PTEN gene may induce denaturalization, necrosis, and apoptosis of the highly metastatic mucoepidermoid carcinoma cells.
【译】KRAS基因密码子12和13与无病生存的野生型BRAF的II I期结肠癌辅助化疗试验从一个突变(n0147联盟)。
KRAS codon 12 and 13 mutations in relation to disease-free survival in BRAF-wild type stage III colon cancers from an adjuvant chemotherapy trial (N0147 Alliance).
目前野生型、先天缺陷型、基因修饰等动物模型被广泛应用在这一研究领域。
In current, different kinds of methods were widely used in the animal model, such as the wild type, congenital defect and gene modification.
经蛋白免疫印记法证实，与同窝野生型小鼠相比，转基因小鼠的脑组织和肌肉组织中TRPV1的表达显著升高。
As detected by Western blotting, TRPV1 expressions in the brain and skeletal muscle of the transgenic mice were significantly higher than the wild-type littermates.
他们将原癌基因诱导的淋巴瘤移植到野生型小鼠，然后在淋巴瘤细胞启动p53表达。
They transplanted Myc-driven lymphoma cells into wild-type mice and then turned on p53 expression in the lymphoma cells.
干旱和盐胁迫导致野生型和转基因型植株丙二醛含量升高，但野生型植株的丙二醛含量增加量要远远高于转基因植株；
MDA contents of both transgenic and wild type plants increased under the drought and high-salt stresses, but MDA contents in wild type plants increased much more than that in the transgenic plants.
在几个番茄茸毛突变体与对应的野生型中，这个基因表达量存在明显差异。
Also significant differences of ShMYB1 expression were detected between several trichome mutants and their wild types.
更重要的是，与野生型相比，转基因植株在长期的渗透胁迫下会积累更高的鲜重，同时在干旱胁迫中能够保留更多的水分。
Importantly, transgenic plants accumulated higher fresh weight under long-term osmotic stress, and also have shown retention of more water than the wild type during drought stress.
PML野生型和基因敲除细胞对抗癌药物LDM的反应提示正常细胞和癌细胞对抗肿瘤药物反应过程中PML蛋白不是唯一的途径。
The reaction of anticancer drug LDM with PML-wild-type and-knockout cells may elucidate that its not the unique pathway in the mechanism of LDM's action on normal and cancer cells.
PML野生型和基因敲除细胞对抗癌药物LDM的反应提示正常细胞和癌细胞对抗肿瘤药物反应过程中PML蛋白不是唯一的途径。
The reaction of anticancer drug LDM with PML-wild-type and-knockout cells may elucidate that its not the unique pathway in the mechanism of LDM's action on normal and cancer cells.
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