重组质粒表达产物定位于细胞核；
Expression products of recombinant plasmid mainly located in nucleus.
用化学法把重组质粒转化到E 。
筛选出干扰效率最高的重组质粒。
In the end filter out the most interference efficient plasmid.
然后阳性重组质粒转化入e。
经静脉分别注入重组质粒及空白质粒。
And then the recombinant plasmid and empty plasmid was delivered by intravenous injection.
重组质粒的转化及筛选；
用酶切和PCR的方法对重组质粒进行鉴定。
Used restrict enzyme and PCR to verify the reconstructed plasmid.
目的：建立一种快速筛选阳性重组质粒的方法。
Objective: To find a rapid method of screening positive recombinant plasmid.
目的构建大肠杆菌双杂交系统的诱饵重组质粒。
Objective To construct a bait plasmid in bacterial two-hybrid system.
本发明还提供了该重组质粒的制备方法和用途。
The invention also provides the preparative method and usage of the recombination plasmid.
经pcr和测序的方法鉴定重组质粒是否构建成功。
Tto identify whether the construction of recombinant plasmid. is successful by PCR and sequencing.
目的构建弓形虫棒状体蛋白2 （ROP2 ）基因重组质粒。
Objective To construct a recombinant plasmid containing rhoptry protein 2 (ROP2) gene of Toxoplasma gondii .
重组质粒经酶切和测序证实正确，然后转化大肠杆菌BL21。
The recombinant plasmid was confirmed by digestion and sequence, then transformed into E. coli BL21.
对含人EC-SOD重组质粒的工程菌进行诱导表达形成包涵体。
The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body.
目的：构建HPV16L1-E7C重组质粒并研究其细胞免疫性。
Objective:To construct the recombinant plasmid of HPV16L1-E7C and study its cellular immunity.
重组质粒PSP1，转化微生物，产生一种碱性蛋白酶vapk的方法。
Recobinant plasmid PSP1, microorganisms transformed therewith, and method for producing alkaline protease VAPK.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chain termination method.
重组质粒pdsbcm，转化微生物，产生一种碱性蛋白梅vapk的方法。
Recombinant plasmid PDSBCM, microorganisms transformed therewith, and method for producing alkaline protease VAPK.
其中，仅S和S1蛋白重组质粒能够诱导中和抗体的产生，以S蛋白的效价为高。
Of three forms of S recombinant proteins, only S or S1 protein induced neutralizing antibody responses, antibody titer was increased by S protein immunization.
用这3种重组质粒分别转染293t细胞，蛋白表达经蛋白质印迹检测得到确认。
The expression of the above three HA DNA vaccines was verified in 293t cells by Western blot analysis.
免疫组化染色后在重组质粒注射部位肌肉组织的肌纤维内显示阳性的棕黄色分泌颗粒。
The specific masculine brown-yellow color reaction was seen in the muscle fibers of recombinant plasmid injected group.
目的：探索优化重组质粒转染细胞后的筛选方法，以得到高纯度U 937转染细胞。
Objective to optimize the selective method to obtain the pure recombinant plasmid transfected U937 cells.
结果酶切鉴定和DNA测序分析显示，TROP2靶向RNA干扰重组质粒构建成功。
Results Enzyme digestion analysis and DNA sequencing showed that TROP2 targeted RNA interference recombinant plasmids were constructed successfully.
该免疫毒素质粒的制备方法包括重组质粒的构建、重组质粒转染的工程菌的制备等步骤。
The preparation process of the immunotoxin plasmid includes the construction of the recombinant plasmid, the preparation of the recombinant transinfected engineering bacterium and other steps.
例如，早期的一次经验让我去深刻认识到遗传学是一个包括重组质粒的组建和构造的工程。
For example, one experience that early on convinced me to seriously consider genetics was a project involving the construction and characterization of a recombinant plasmid.
目的筛选肝再生增强因子重组质粒基因治疗时较优的载体、途径和剂量及三者的最佳组合。
Objective To select an optimal combined set of carrier, introducing route and dose of recombinant plasmid of augmenter of liver regeneration (ALR) for ALR gene therapy.
我们将对此重组质粒在包装细胞（PT67）的扩增以及对靶细胞转导的效应作进一步研究。
The proliferation of recombinant plasmid in packaging cells (PT67) would be studied further as well as the transducing effect to the target cells.
目的：研究DT390-IL2重组质粒对实验性变态反应性脑脊髓炎（EAE）的治疗效果。
Objective:To study the curative effect of EAE with DT390-IL2 recombinant plasmid.
尽管如此，这些重组质粒仍可用做DNA指放探针，在有关动物上产生个体特异性的DNA指纹图。
Nevertheless, these recombinant plasmids could be used as DNA fingerprinting probes and produced individual-specific DNA fingerprints plasmids in several animal species.
尽管如此，这些重组质粒仍可用做DNA指放探针，在有关动物上产生个体特异性的DNA指纹图。
Nevertheless, these recombinant plasmids could be used as DNA fingerprinting probes and produced individual-specific DNA fingerprints plasmids in several animal species.
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