将纯化的在大肠杆菌中表达的鹦鹉热衣原体(Cps)的重组主要外膜蛋白(MOMP)与油佐剂混合。
The purified recombinant major outer-membrane protein (MOMP) of Chlamydia psittaci (Cps) expressed in E. coli was mixed with oil adjuvant.
重组伪狂犬病毒构建成功,且表达的ORF2蛋白具有免疫原性。
The recombinant pseudorabies virus was constructed successfully, and the expressed ORF2 protein had immunogenicity.
间接免疫荧光、ELISA和免疫印迹检测证明,含SO.7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
目的:合成胆汁三烯结合蛋白(BBP)基因并在大肠杆菌中表达,获得重组BBP纯化制品。
Objective: To synthesize bilin binding protein (BBP) gene sequence, express BBP efficiently in Escherichia coli and purify the recombinant protein.
目的观察周围神经修复后,重组睫状神经营养因子(CNTF)对相关神经元中生长相关蛋白表达的调控作用。
Objective to study the effects of recombinant ciliary neurotrophic factor (CNTF) on expression of growth related proteins in related neurons after sciatic nerve repair in rats.
目的:构建弓形虫棒状体蛋白(ROP2 )基因重组质粒并在大肠杆菌中表达,用于筛选弓形虫新的诊断抗原和疫苗分子。
Objective:To construct a recombinant plasmid containing rhoptry protein 2(ROP2)gene of Toxoplasma gondii and express it in E. coli for selection of diagnostic antigen and vaccine candidate.
此重组体可在酵母中进一步表达特异性蛋白。
The recombination EgB can be used for the expression of specific protein in yeast.
目的:探索重组人胎盘抗凝蛋白变体大肠杆菌表达菌株的最适表达条件。
Objective the purpose of this study is to optimize the different parameters in the expression of recombinant human placental anticoagulant protein derivative expressing e.
表达产物用亲和层析一步法进行纯化,然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
试剂盒采用原核表达的重组P30蛋白作为包被抗原,依据竞争ELISA原理检测猪血清中非洲猪瘟病毒的抗体。
The kit adopts prokaryotic expression recombinant P30 protein as coating antigen, and detects the African swine fever virus antibody in pig serum according to a competitive ELISA principle.
本发明还涉及用于在宿主中表达这些功能性结合蛋白的重组载体。
The present invention further relates to recombinant vectors useful for the expression of these functional binding proteins in a host.
完成了NKG2D的原核表达载体的构建,表达并纯化了重组NKG2D蛋白。
The NKG2D prokaryotic expression vector was successfully constructed. The recombinant NKG2D is expressed and purified.
本文实现了重组别藻蓝蛋白基因在毕赤酵母中的异源表达。
In this paper, the heterogeneous expression of recombinant allophycocyanin in Pichia pastoris was completed.
试剂盒中96孔板中的包被抗原为原核表达的重组P 30蛋白,其具有良好的抗原性。
The coating antigen in a 96 pore plate in the kit is the prokaryotic expression recombinant P30 protein, and has good antigenicity.
目的:考察原核表达的重组人NDRG2蛋白对肿瘤细胞的作用。
Objective: To study the effects of prokaryotically expressed recombinant human NDRG2 on tumor cell lines.
利用鹦鹉热衣原体重组主要外膜蛋白(MOMP)在大肠杆菌中的表达产物,纯化后与白油佐剂乳化成疫苗。
The purified recombinant major outer-membrane protein (MOMP) of Chlamydia psittaci(Cps)expressed in E. coli was mixed with oil adjuvant and manufactured as MOMP-vaccine.
方法在原核表达系统中高效表达重组人3型腺病毒六邻体蛋白。
Methods Under the optimum expression conditions, the hexon protein of human type 3 adenovirus was efficiently expressed in e.
介绍了近年来重组蛋白质的常用表达系统以及各种分离纯化技术等研究和应用的进展情况。
The study and application were introduced on the expression system in common use and separation and purification technique of recombinant proteins in recent years.
实现其在CHO - DHFR -细胞中的高效表达,获取生物活性较高的重组蛋白。
AIM: TO obtain high level expression of recombinant human truncated osteoprotegerin (TOPG) with higher bioactivity in CHO-DHFR-cells.
目的利用杆状病毒表达载体系统制备重组乙型肝炎病毒核心蛋白。
Objective To produce recombinant HBcAg in insect cells by Baculovirus expression vector system.
对大肠杆菌表达的重组人胰岛素原包涵体蛋白的变性复性条件进行了优化。
Optimization of the renaturation conditions for recombinant human proinsulin inclusion bodies expressed in E.
目的高密度培养重组细菌和高效表达重组幽门螺杆菌尿素酶a融合蛋白。
Cell density cultivation and high level expression of recombinant urease a fusion protein in Helicobacter pylori.
结论克隆BLCAP基因并重组原核细胞表达质粒,为分析蛋白特性、研究基因功能创造了条件。
Conclusions: it is very helpful to clone and construct the recombined plasmid of BLCAP gene and to study the qualities of protein and the function of BLCAP gene.
设计了一种新的病原体蛋白质B细胞抗原表位的筛选和重组表达方法。
A novel method was designed for disease specific B cell epitope mapping and epitope expression in e.
结论:用硫氧还蛋白融合表达系统在大肠杆菌中表达的小鼠内皮抑素重组融合蛋白易纯化并具有高活性。
CONCLUSION: The mouse endostatin recombinant fusion protein expressed in E. coli by using thioredoxin fusion expression system is easy to be purified and possesses high activity.
苦荞麦主要过敏蛋白N端基因的获得为该蛋白的重组表达及其生物学活性等研究建立了前期基础。
The obtain- ment of the N-terminal gene of major allergenic protein in tartary buckwheat should lay an important foundation for further study of the tartary buckwheat allergen.
综述了重组人血清白蛋白的性质、结构和功能,及常用表达系统。
The structure, the main function and the expression systems of recombinant human serum albumin were reviewed in this article.
重组蛋白以包涵体的形式进行表达。
Expressed fusion proteins existed in the form of inclusion body.
重组蛋白以包涵体的形式进行表达。
Expressed fusion proteins existed in the form of inclusion body.
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