蛋白印迹实验鉴定转基因植株，ELISA方法测定重组蛋白表达水平，集落形成实验测定重组蛋白活性。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
表达产物用亲和层析一步法进行纯化，然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
介绍了近年来重组蛋白质的常用表达系统以及各种分离纯化技术等研究和应用的进展情况。
The study and application were introduced on the expression system in common use and separation and purification technique of recombinant proteins in recent years.
实现其在CHO - DHFR -细胞中的高效表达，获取生物活性较高的重组蛋白。
AIM: TO obtain high level expression of recombinant human truncated osteoprotegerin (TOPG) with higher bioactivity in CHO-DHFR-cells.
将工程菌发酵培养，经诱导、表达获得重组蛋白。
Said engineering bacterium is undergone the processes of fermentation, culture, induction, expression to obtain recombinant protein.
重组蛋白以包涵体的形式进行表达。
Expressed fusion proteins existed in the form of inclusion body.
SDS电泳分析结果表明EPSPS重组蛋白的表达量在55%以上，纯化的EPSPS重组蛋白纯度可以达到90%。
The SDS gel electrophoresis analysis shows that the expression of EPSPS recombined protein is over 55% and the purified EPSPS recombined protein reached as 90%.
目的在大肠埃希菌中表达人神经元正五聚蛋白2 (NPTX2)，并对该重组蛋白进行纯化、鉴定。
Objective to construct the prokaryotic expression vector of human neuronal pentraxin 2 (NPTX2) gene, induce the expression of the recombinant fusion protein in e.
结果：研究中克隆表达的重组蛋白序列与公布的SARS病毒S1蛋白的序列相同。
Results:Sequencing analysis confirmed that the recombinant protein has the same sequence of natural SARS virus S1 subunit.
目的构建人高迁移率族蛋白1（HMGB1）编码基因的表达载体，获得纯化的重组蛋白，研究其生物学功能。
Objective To construct the recombinant vector, the expression of human HMGB1 code gene and to study its function.
重组蛋白技术在许多领域已成为一种必要的工具，但有时表达的目的蛋白却未进行正确的折叠而被蛋白酶降解或聚集形成包涵体。
Many recombinant proteins fail to fold into native state with activity when expressed in Escherichia coli, instead, they often misfold and are easy to accumulate as inclusion bodies.
结果表明重组蛋白只在沉淀中表达即以包涵体形式存在。
To test whether AtADCL in Arabidopsis thaliana has enzymatic activity, soluble form of the recombinant protein has to be obtained.
目的构建人鸟氨酸脱羧酶抗酶(OAZ)突变基因，重组至原核表达载体中并表达出重组蛋白。
Objective To clone human ornithine decarboxylase antizyme (OAZ) mutation gene and express its recombinant protein.
结果构建了具有正确基因序列的CFP10重组表达质粒，重组蛋白在大肠杆菌BL21（DE3）中以包涵体形式表达。
The recombinant CFP10 protein was expressed in inclusion body in E. coli BL21(DE3), and the target gene had been cloned into host bacterium.
目的克隆人促甲状腺激素受体胞外段基因，构建重组真核表达质粒，获得具有免疫学活性的纯化重组蛋白。
Objective to clone and construct the plasmid containing human thyroid stimulating hormone receptor (TSHR) gene ectodomain, and then identify the immunoreactivity of the purified recombinant protein.
兔淋巴母细胞增殖实验证明转染后，表达的重组蛋白能够促兔外周淋巴细胞增殖作用，具有一定的生物学活性。
The expression products also to increase the proliferation of lymphoblast from the blood of rabbit, which has some biological activities.
重组蛋白在大肠杆菌中表达多为无活性的包涵体形式，须经洗涤、溶解、复性后才能得到生物活性蛋白。
Expression of recombinant proteins in Escherichia coli often results inclusion bodies, an aggregation form of inactive proteins.
分别以重组蛋白疫苗、商品疫苗和PBS免疫仔猪，优化检测免疫抗体水平的间接ELISA条件，通过比较三个免疫组的抗体水平来评价表达蛋白作为免疫抗原的有效性。
Piglets of each groups were vaccinated respectively with recombinant protein vaccine, commodity vaccine and PBS as contrast. The serum IgG anti-protein of each groups were detected by indirect ELISA.
结论：原核表达载体的构建、重组蛋白的表达、纯化及多克隆抗体的制备为今后研究该蛋白的功能提供了良好的基础。
CONCLUSION: Recombined prokaryotic expression vector, the purified protein and prepared polyclonal antibody were the necessary materials for further study of this protein.
利用镍柱亲和层析对表达的重组蛋白进行纯化，经SDS-PAGE及薄层扫描分析表明，重组蛋白的纯度在90%以上；
The recombinant protein was purified through Ni-chelating affinity chromatography, and the purity was above 90% explained by SDS-PAGE gel scan analysis.
HEV结构区ORF2蛋白在甲醇营养型酵母中的成功表达，以及初步纯化得到的具有强免疫学活性的重组蛋白，为研制新型戊型肝炎基因工程疫苗奠定了基础。
The successful expression of HEV ORF2 protein in p. Pastoris and the production of recombinant protein provides basis for genetically engineered vaccine development of hepatitis E.
人们利用莱茵衣藻叶绿体表达体系已成功表达多种重组蛋白，其中包括人类药用蛋白。
The C. reinhardtii chloroplast foreign gene expression system has been utilized successfully to produce a variety of recombinant proteins including human therapeutic proteins.
对重组蛋白的氨基酸组分分析表明重组表达的真菌免疫调节蛋白具有和天然蛋白相同的氨基酸组分。
The amino acid composition analysis showed that the recombinant FIPs had similar amino acid composition to the nature ones.
为了获得大量有活性的重组蛋白酶便于今后的工业应用，我们将蛋白酶WF146克隆到分泌性表达宿主枯草芽胞杆菌和巴斯德毕赤酵母中。
For the convenience of production and purification, Bacillus subtilis and Pichia pastor is were used as hosts for the expression of protease WF146.
为了获得大量有活性的重组蛋白酶便于今后的工业应用，我们将蛋白酶WF146克隆到分泌性表达宿主枯草芽胞杆菌和巴斯德毕赤酵母中。
For the convenience of production and purification, Bacillus subtilis and Pichia pastor is were used as hosts for the expression of protease WF146.
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