该单克隆抗体是通过用重组蛋白免疫动物而制备的，该重组蛋白是人FGF受体2蛋白羧基末端区域的残基。
Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to residues near the carboxy terminal region of human FGF receptor 2 protein.
该单克隆抗体是通过用重组蛋白免疫动物而制备的，该重组蛋白是人FGF受体1蛋白的羧基末端。
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human FGF receptor 1 protein.
该单克隆抗体是通过用重组蛋白免疫动物而制备的，该重组蛋白是针对人FGF受体1蛋白的羧基末端。
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human FGF receptor 1 protein.
该单克隆抗体是通过用重组蛋白免疫动物而制备的，该重组蛋白包含人VEGF受体2蛋白羧基末端150氨基酸残基。
Monoclonal antibody is produced by immunizing animals with a recombinant protein containing the carboxy-terminal 150 amino acid residues of human VEGF receptor 2 protein.
该单克隆抗体通过用重组蛋白免疫动物制备，该重组蛋白是人egfr1蛋白胞外结构域附近的残基。
Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to the extracellular domain of human EGFR1 protein.
同时，利用此重组蛋白为抗原，制备了小鼠组织因子的小鼠源功能阻断性单克隆抗体，在血浆凝集实验中证明其对小鼠组织因子的活性有明显抑制作用。
Using this recombinant mTF as immunogen, a murine monoclonal function-blocking antibody to mTF was also generated, which can inhibit mTF-initiated plasma clotting in vitro.
结论：原核表达载体的构建、重组蛋白的表达、纯化及多克隆抗体的制备为今后研究该蛋白的功能提供了良好的基础。
CONCLUSION: Recombined prokaryotic expression vector, the purified protein and prepared polyclonal antibody were the necessary materials for further study of this protein.
通过裂解、洗涤、变性、复性等方法对包涵体蛋白进行处理，以电泳切胶回收所获得的纯化产物作为抗原，免疫小鼠制备抗重组蛋白血清。
The recombination fusion protein was purified by lysis, washing, denaturation and renaturation. The specific band of expression was excised from the gel and used to immunize mice .
用本发明方法制备的猴金属硫蛋白I的六个突变体蛋白，产量与纯度均与野生型重组蛋白类似。
Using the said method to prepare 6 mutant proteins of monkey metallothionein I can obtain yield and purity similar to that in wild recombinant protein.
用本发明方法制备的猴金属硫蛋白I的六个突变体蛋白，产量与纯度均与野生型重组蛋白类似。
Using the said method to prepare 6 mutant proteins of monkey metallothionein I can obtain yield and purity similar to that in wild recombinant protein.
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