所述病毒优选是重组腺病毒。
同时观察重组腺病毒对荷瘤小鼠的治疗效应。
Meanwhile, the therapeutic effect of the recombinant adenovirus was also observed in tumor-bearing mice.
目的通过细菌内同源重组方法构建重组腺病毒。
Objective To construct recombinant adenovirus by homologous recombination in bacteria.
该重组腺病毒可以正确表达目的蛋白，效价高。
The recombinant adenovirus can correctly express a target protein and has high potency.
目的构建含有人TRADD基因片段的重组腺病毒载体。
Objective to construct recombinant adenovirus vector carrying human TRADD gene.
结论可以利用阳离子脂质体来提高重组腺病毒的转染效率。
CONCLUSION Lipofectamine may be used as a tool to enhance the efficacy of transfection with recombinant adenovirus.
目的体外构建和表达可调控性人抑癌基因pten重组腺病毒。
Objective to construct and express a recombinant adenovirus vector which can regulate expression of human tumor suppressor gene PTEN.
目的利用细菌内同源重组法构建含ICOS胞外区基因的重组腺病毒。
Objective To construct the recombinant adenovirus of human ICOS extracellular domain gene by using the method of homologous recombination in bacteria.
目的探讨表皮生长因子受体反义重组腺病毒联合放射线对乳腺癌细胞的作用。
Objective to investigate the effects of a recombinant antisense adenovirus for epidermal growth factor receptor combined with irradiation on breast cancer cells.
以不同感染强度的携带绿色荧光蛋白基因重组腺病毒转染诱导后的脂肪细胞。
The adipose cells were transfected with Ad-GFP at the different multiplicity of infection (MOI).
同时制备裸鼠u251细胞移植瘤模型并注射重组腺病毒，观察肿瘤生长情况。
The recombinant viruses were injected into the xenograft tumors of U251 cells in nude mice, then the tumor growth was observed.
目的制备表达人单核细胞趋化蛋白- 1 (MCP - 1)复制缺陷型重组腺病毒。
Objective To construct replication deficient recombinant adenovirus of human monocyte chemoattractant protein-1 (MCP-1) by homologous recombination.
目的探讨大鼠心脏移植过程中，重组腺病毒介导的外源基因转移至供心的可行性及安全性。
Objective to study efficiency and security of the recombinant adenoviral-mediated gene transfer to the donor heart during the heart transplantation.
目的研究联合运用狂犬病毒糖蛋白基因重组腺病毒与同一抗原核酸疫苗对小鼠的免疫效果。
Objective To investigate the immune response of mice primed by DNA particle bombing and boosted by immunization with replication-defective adenoviral recombinant bearing rabies glycoprotein gene.
结果：与野生型腺病毒相比，重组腺病毒能显著地提高目的基因在四种不同肿瘤细胞中的表达。
Results Fiber-mutant adenovirus vector showed a notably enhanced gene expression in four different tumor cells compared with that of conventional ones.
目的：建立一种在甲胎蛋白(afp)阳性的肝癌细胞中靶向表达目的基因的重组腺病毒载体。
AIM: to construct a recombinant adenovirus vector carrying AFP promoter to specifically express a targeting gene in hepatocellular carcinoma HepG2 cells.
目的构建携带反义热休克蛋白70 (HSP70)的重组腺病毒载体以用于喉癌的基因治疗研究。
Objective to construct a recombinant adenovirus vector carrying antisense heat shock protein 70 (HSP70) cDNA and observe its effect on inhibiting the growth of laryngeal carcinoma Hep-2 cells.
结论成功地构建了携带CCR5反义rna重组腺病毒载体，为研究其抗hiv 1的作用打下基础。
Conclusion The recombinant adenovirus carrying antisense RNA to CCR5 has laid down a good foundation for studying its inhibiting effect on HIV-1 infection.
目的：构建含人IL -21基因的重组腺病毒表达载体，为IL - 21基因治疗肿瘤研究奠定基础。
Objective: to be as the foundation of the tumor therapy with targeting IL-21 gene by constructing of recombinant interleukin 21 gene adenovirus expression vector.
目的：探讨小鼠心脏移植中，通过在体外保存期间对供心进行灌注，进行重组腺病毒基因转染的方法及效率。
AIM: To establish a method of in vitro donor heart perfusion in murine cardiac transplantation during preservation and apply it in adenovirus mediated gene transfection for donor heart.
本发明还涉及人源化改造的基因突变技 术和去除GFP的腺病毒转移载体及去除GFP的同源重组腺病毒载体。
The invention also relates to a human reforming gene mutation technique, adenovirus transition carrier removing GFP and homologous recombinant adenovirus carrier.
目的：构建表达小鼠轮状病毒（EDIM）EW株VP7基因的重组腺病毒，以在小鼠模型上研究轮状病毒的免疫保护机制。
Objective:To construct a replication defective recombinant adenovirus expressing murine rotavirus(EDIM)EW strain VP7to recruit it in studies on the protective immunization of rotavirus.
结论构建成功的含有TRADD基因片段的重组腺病毒，可用于转染人成纤维细胞，用于检测TRADD对病理性瘢痕成纤维细胞的影响。
Conclusion the recombinant adenovirus vector carrying human TRADD gene was successfully constructed. It can be used for detecting the effects of TRADD on fibroblasts of hypertrophic scar and keloid.
目的：构建趋化因子受体c CR 5,CXCR4双靶区反义rna重组载体并获取重组腺病毒以用于抗hiv1基因治疗的研究。
AIM: to construct the recombinant adenoviral vector carrying antisense RNA to chemokine receptors CCR5 and CXCR4 and to obtain recombinant adenovirus, which will be used to resist HIV 1 infection.
利用基因重组原理，构建可表达CNTF的腺病毒。
The CNTF recombinant Adenovirus was constructed using the Adenovirus system.
结论利用位置特性重组技术，成功的构建了人反义B7 - H1的腺病毒载体，为后续对B7 - H1的相关研究创造了条件。
Conclusion the recombinant adenovirus vector was constructed successfully by site-specific recombination technique, which provides a foundation for further research about B7-H1.
目的获得具有感染性的狂犬病病毒核蛋白犬2型腺病毒，并对重组病毒生物学和免疫学特性进行初步探讨。
Objective to construct a rabies virus nucleoprotein containing canine adenovirus type 2 (CAV-2) recombinant, and to evaluate the biological and immunological properties of the recombinant virus.
方法在原核表达系统中高效表达重组人3型腺病毒六邻体蛋白。
Methods Under the optimum expression conditions, the hexon protein of human type 3 adenovirus was efficiently expressed in e.
方法在原核表达系统中高效表达重组人3型腺病毒六邻体蛋白。
Methods Under the optimum expression conditions, the hexon protein of human type 3 adenovirus was efficiently expressed in e.
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