目的：构建及鉴定载入金属蛋白酶组织抑制因子1（TIMP鄄1）的重组腺相关病毒载体。
Objective: To construct and identify recombinant adeno-associated virus encoding rat tissue inhibitor of metalloproteinase-1(TIMP-1) full length cDNA.
同时在连续传代40代后每隔5代提取重组病毒全基因组进行酶切鉴定，发现其酶切位点保持不变。
To evaluate its heredity stability, the recombinant virus genome was extracted and digested by Hindlll every 5 generations, and 40 generations later, its enzyme digestion sites almost keep stable.
近年来杆状病毒介导的昆虫表达系统得到了广泛地应用，本课题通过该系统表达CYP2B6重组酶，并进行活性鉴定。
In this study, CYP2B6 recombinant enzyme was obtained using baculovirus expression system and its catalytic activity was measured using bupropion as probe.
经鉴定证实目的基因发生了特异转座和获得的病毒为特异的重组病毒。
It was approved that the gene transposition and specific virus recombination were performed from the constructed plasmids.
经鉴定证实目的基因发生了特异转座和获得的病毒为特异的重组病毒。
It was approved that the gene transposition and specific virus recombination were performed from the constructed plasmids.
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