重组病毒载体因其自然感染途径而成为有效的基因转移手段。
Recombinant viral vectors have become effective tools for gene transfer by means of their natural infective ways.
目的：构建抗腮腺炎病毒抗体轻链基因重组表达载体。
Objective: To construct the recombinant expression vector of genes encoding light chain of antibody against Mumps Viruses.
目的探讨重组杆状病毒作为哺乳动物基因转导载体的可行性及其转导特点。
Objective to study the feasibility and the characteristics of recombinant baculovirus as mammalian gene transfer vector.
该研究结束时，在细胞移植和为基因传输所进行的重组细胞病毒载体研发这两方面取得了进展。
To this end, advances have been made in the areas of cell transplantation and in the development of recombinant viral vectors for gene delivery.
目的利用杆状病毒表达载体系统制备重组乙型肝炎病毒核心蛋白。
Objective To produce recombinant HBcAg in insect cells by Baculovirus expression vector system.
目的：构建及鉴定载入金属蛋白酶组织抑制因子1（TIMP鄄1）的重组腺相关病毒载体。
Objective: To construct and identify recombinant adeno-associated virus encoding rat tissue inhibitor of metalloproteinase-1(TIMP-1) full length cDNA.
结论利用位置特性重组技术，成功的构建了人反义B7 - H1的腺病毒载体，为后续对B7 - H1的相关研究创造了条件。
Conclusion the recombinant adenovirus vector was constructed successfully by site-specific recombination technique, which provides a foundation for further research about B7-H1.
探讨重组2型腺相关病毒载体介导2型成纤维细胞生长因子基因诱导家兔缺血心肌血管生成的作用。
Aim To investigate the effect of fibroblast growth factor 2 (FGF2) cDNA mediated by recombinant adeno-associated viral vector2 (rAAV-2) on ischemic myocardium.
杆状病毒载体将在未来药物研发、疫苗生产、基因治疗、重组杆状病毒杀虫剂等领域得到广泛应用。
The insect baculovirus, in the new century, will be applied in drug research and development, production of vaccines, gene therapy and recombinant baculovirus insecticides.
结果：成功地构建了重组BMP7逆转录病毒表达载体，并可在骨髓基质干细胞中表达BMP 7。
The viruses were used to infect directly BMSCs and the expression of BMP 7 gene in BMSCs was analyzed by immunohistochemical staining.
目的：构建含人IL -21基因的重组腺病毒表达载体，为IL - 21基因治疗肿瘤研究奠定基础。
Objective: to be as the foundation of the tumor therapy with targeting IL-21 gene by constructing of recombinant interleukin 21 gene adenovirus expression vector.
结论构建了含人pd - 1基因重组逆转录病毒的载体，筛选出稳定表达人PD - 1分子的L929细胞株。
Conclusion HumanPD-1 gene has been cloned, recombinant retrovirus vector containing PD-1 gene constructed and L929 transfection cell line expressing PD-1 molecules selected.
结论成功地构建了携带CCR5反义rna重组腺病毒载体，为研究其抗hiv 1的作用打下基础。
Conclusion The recombinant adenovirus carrying antisense RNA to CCR5 has laid down a good foundation for studying its inhibiting effect on HIV-1 infection.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
本发明还涉及包含所述非病毒载体和所述表达型重组体的药物组合物及其应用。
The invention also relates to the pharmaceutical compositions containing the non-viral vectors and the expression type recombinant and their use.
目的构建反义及正义波形蛋白重组逆转录病毒载体，研究波形蛋白在反应性胶质化中的功能与作用。
Objective to construct antisense and sense vimentin retrovirus vector for investigating the effect of vimentin on reactive gliosis.
重组人胰岛素基因逆转录病毒载体质粒应用于活体动物是安全的。
The application to rats of recombinant human insulin gene retroviral vector plasmid mutated in three place with physiological regulatory element constructed by us was safe.
目的：构建趋化因子受体c CR 5,CXCR4双靶区反义rna重组载体并获取重组腺病毒以用于抗hiv1基因治疗的研究。
AIM: to construct the recombinant adenoviral vector carrying antisense RNA to chemokine receptors CCR5 and CXCR4 and to obtain recombinant adenovirus, which will be used to resist HIV 1 infection.
目的构建携带反义热休克蛋白70 (HSP70)的重组腺病毒载体以用于喉癌的基因治疗研究。
Objective to construct a recombinant adenovirus vector carrying antisense heat shock protein 70 (HSP70) cDNA and observe its effect on inhibiting the growth of laryngeal carcinoma Hep-2 cells.
目的构建含有人TRADD基因片段的重组腺病毒载体。
Objective to construct recombinant adenovirus vector carrying human TRADD gene.
本发明涉及含有外源基因的重组、嵌合单股负链病毒载体的用途。
The present invention is directed to the use of recombinant, chimeric mononegavirale vectors comprising a foreign gene.
目的：建立一种在甲胎蛋白(afp)阳性的肝癌细胞中靶向表达目的基因的重组腺病毒载体。
AIM: to construct a recombinant adenovirus vector carrying AFP promoter to specifically express a targeting gene in hepatocellular carcinoma HepG2 cells.
本发明公开了一种重组犬2型腺病毒转移载体、其构建方法及其用途。
The present invention discloses a recombinant canine adenovirus-2 transfer vector, a method for constructing the recombinant canine adenovirus-2 transfer vector and an applications thereof.
本发明还涉及人源化改造的基因突变技 术和去除GFP的腺病毒转移载体及去除GFP的同源重组腺病毒载体。
The invention also relates to a human reforming gene mutation technique, adenovirus transition carrier removing GFP and homologous recombinant adenovirus carrier.
本发明还涉及人源化改造的基因突变技 术和去除GFP的腺病毒转移载体及去除GFP的同源重组腺病毒载体。
The invention also relates to a human reforming gene mutation technique, adenovirus transition carrier removing GFP and homologous recombinant adenovirus carrier.
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