经同源重组后，以终点稀释和斑点杂交法筛选重组杆状病毒；
After co-transfection of recombinant plasmid and wild type AcNPV DNA, the rAcNPV was confirmed by the end point dilution assay and dot blot.
目的探讨重组杆状病毒作为哺乳动物基因转导载体的可行性及其转导特点。
Objective to study the feasibility and the characteristics of recombinant baculovirus as mammalian gene transfer vector.
然后使用重组杆状病毒技术使这些S1亚单位在昆虫细胞和昆虫幼虫中实现了高水平表达。
The modified S1 subunits were abundantly expressed by recombinant autographa californica nuclear polyhedrosis viruses in insect cells and larva.
杆状病毒载体将在未来药物研发、疫苗生产、基因治疗、重组杆状病毒杀虫剂等领域得到广泛应用。
The insect baculovirus, in the new century, will be applied in drug research and development, production of vaccines, gene therapy and recombinant baculovirus insecticides.
间接免疫荧光、ELISA和免疫印迹检测证明，含SO.7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
近年来杆状病毒介导的昆虫表达系统得到了广泛地应用，本课题通过该系统表达CYP2B6重组酶，并进行活性鉴定。
In this study, CYP2B6 recombinant enzyme was obtained using baculovirus expression system and its catalytic activity was measured using bupropion as probe.
目的利用杆状病毒表达载体系统制备重组乙型肝炎病毒核心蛋白。
Objective To produce recombinant HBcAg in insect cells by Baculovirus expression vector system.
结论杆状病毒表达系统能成功表达有活性的CYP2B6重组酶，可用来进一步研究在药物代谢中的作用。
CONCLUSION Active recombinant human CYP2B6 obtained using baculovirus expression system will facilitate further examination of the role of this enzyme in drug metabolism.
结论杆状病毒表达系统能成功表达有活性的CYP2B6重组酶，可用来进一步研究在药物代谢中的作用。
CONCLUSION Active recombinant human CYP2B6 obtained using baculovirus expression system will facilitate further examination of the role of this enzyme in drug metabolism.
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