方法：用重组工程技术来快速高效地构建条件敲除载体。
Methods: By recombineering technique, construct conditional knockout vector rapidly and efficiently.
“位点专一重组酶的优势就是酶做了所有的事。”斯达克说，他同时还在调查研究Z FR在遗传工程学中的潜力。
"The advantage of a site-specific recombinase is that the enzyme does everything," says Stark, who is also investigating the potential of ZFRs in genetic engineering.
进行这种金融工程，即所谓的股息资本重组的不只thl一家。
THL was hardly alone in undertaking this sort of financial engineering, known as a dividend recapitalization.
简述近年来重组受体、转基因动物、基因探针等基因工程技术在药物筛选中的应用。
The recent application of recombinant receptors, transgenic animals, genetic probe and other genetic engineering technology in drugs screening is introduced briefly.
细胞工程主要包括体细胞融合，核移植，细胞器摄取和染色体片段的重组等。
The works mainly comprise the body cells, cell fusion, nuclear transplantation, cell uptake and the reorganization of chromosome segment.
目的：用基因工程方法制备重组人zp3蛋白。
Objective: To preparation of recombinant human ZP3 protein by gene engineering.
正在应用的或处于研究阶段的构建瘤胃基因工程菌的方式主要有基因缺失技术、基因复制性重组技术和启动子的应用技术。
The main methods used, or under investigation, for production of rumen gene engineering bacteria are:gene deletion technique, gene recombination technique and the application of promotor.
随着重组DNA技术的不断完善，基因工程药物的研制和利用日益增多。
As recombinant DNA technology is consummating increasingly, genetic engineering drug is frequently researched and utilized.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒，稳定高效地获得基因工程产品心钠素。
Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
近年来，基因工程疫苗如重组亚单位疫苗，细菌载体疫苗等新型疫苗的研究具有广泛应用前景。
Nowadays, genetic engineering vaccines such as recombinant subunit vaccines, bacterial deliver system vaccines have an extensive prospect in clinical applications.
代谢工程是指通过某些特定生化反应的修饰来定向改善细胞的特性或运用DN A重组技术创造新的化合物。
Metabolism engineering referred to the improvement of cellular properties by special biochemical reactions or the construction of new compounds by DNA recombination techniques.
例如，早期的一次经验让我去深刻认识到遗传学是一个包括重组质粒的组建和构造的工程。
For example, one experience that early on convinced me to seriously consider genetics was a project involving the construction and characterization of a recombinant plasmid.
对含人EC-SOD重组质粒的工程菌进行诱导表达形成包涵体。
The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body.
目的获得重组3C蛋白酶，开发一种新型的基因工程工具酶。
Objective To obtain human rhinovirus 3C protease as a novel tool enzyme for gene engineering.
本研究工作为CD19-L的生物工程版本（一种重组的人类生物治疗因子，其靶标为CD19阳性白血病干细胞）的首篇报告。
This work represents the first report of a bioengineered version of CD19-L, a recombinant human biotherapeutic agent, targeting CD19-positive leukemic stem cells.
媒体全面报道这一重大事件之后，基因工程和重组DNA的研究成为公众瞩目的焦点。
After the extensive news coverage of this momentous event, the study of genetic engineering and recombinant DNA was thrown into the public spotlight.
重组后的公司业务扩展到食品机械、包装机械和农产品加工工程领域。
After the reorganization the company's business expanded to food machinery, packaging machinery and farm produce processing engineering.
新型竹质工程材料——弧形原态重组竹的开发，为竹材的高效利用提供了一条新的途径。
The development of new bamboo engineering material:recombinant bamboo made from laminated circumferential sections provide a new way for bamboo efficient utilization.
本发明提供了一种重组卡介苗的制备过程，并研究了其免疫性，属于基因工程领域和结核疫苗领域。
The invention provides a process for preparing the recombinant BCG vaccine, researches the immunity thereof, and belongs to the field of gene engineering and the field of tuberculosis vaccine.
目的建立重组NK4融合基因工程菌的发酵工艺。
Objective To develop a fermentation procedure for recombinant E. coli strain expressing NK4 gene.
同时，在有了人手的情况下，我们还急需能刺激并重组巨大自然资源的工程。
At the same time, through this employment, accomplishing greatly needed projects can stimulate and reorganize the use of our great natural resources.
应用基因工程法可以制备出重组降钙素类似物。
By taking advantage of gene engineering, recombinant calcitonin analogue can be successfully obtained.
目的：用基因工程技术生产大量重组CB，为研究CB的生物学特性和制备CB抗体供临床检测作准备。
Aims: To obtain enough recombinant CB by using genetic engineering technique for studying its biological characteristics and obtaining its antibodies for clinical detection.
文化整合是企业重组中一项极易忽视又至关重要的系统工程。
In the realignment of enterprises, cultural integration is easy to be neglected though it is an utmost important systematic engineering.
本试验旨在通过基因工程方法获得重组肌肉生长抑制素蛋白。
This experiment was conducted to gain the recombinant myostatin by technique for gene engineering.
将工程菌发酵培养，经诱导、表达获得重组蛋白。
Said engineering bacterium is undergone the processes of fermentation, culture, induction, expression to obtain recombinant protein.
近年来，国外正在研究如何将工程项目管理与实施的技术和方法应用于新技术开发和信息技术重组。
Techniques and methods for engineering project management and operation have being introduced in the field of new technique development and information system construction in recent years.
测定了猪生长激素基因工程菌不同表达效率时细菌总蛋白的含量以及包涵体中重组猪生长激素占包涵体总蛋白的比值。
We determined the content of the total bacterial proteins and the proportion of recombinant porcine growth hormone(rpGH)in inclusion bodies(IBs)in the cultures of genetically engineered rpGH E.
测定了猪生长激素基因工程菌不同表达效率时细菌总蛋白的含量以及包涵体中重组猪生长激素占包涵体总蛋白的比值。
We determined the content of the total bacterial proteins and the proportion of recombinant porcine growth hormone(rpGH)in inclusion bodies(IBs)in the cultures of genetically engineered rpGH E.
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