利用国产培养基原料在摇瓶发酵中对重组大肠杆菌E。
The fermentation medium of the recombinant bacteria(E. coli BL21) using domestic ingredients was studied.
制得的抗体在重组大肠杆菌里检测到了乙酰转移酶，证明所得抗体具有实验价值。
Acetyltransferase was detected in the E. coli recombinants, which indicates that the antibody is useful for practical studies.
目的建立反相高效液相色谱（RPHPLC）测定重组大肠杆菌发酵液中降血压肽的方法，为其深入研究提供方法学基础。
ObjectiveA simple and rapid reversed-phase high performance liquid chromatographic method was developed for the AHP in Recombinant E. coli fermentation broth.
将纯化的在大肠杆菌中表达的鹦鹉热衣原体（Cps）的重组主要外膜蛋白（MOMP）与油佐剂混合。
The purified recombinant major outer-membrane protein (MOMP) of Chlamydia psittaci (Cps) expressed in E. coli was mixed with oil adjuvant.
当他们把这段重组基因转入到大肠杆菌中去的时候，大肠杆菌即可生产处既定的包含着两种特异氨基酸的蛋白质。
When they inserted the modified gene into E. coli, it produced a modified calmodulin protein, incorporating the two unnatural amino acids.
结论：用硫氧还蛋白融合表达系统在大肠杆菌中表达的小鼠内皮抑素重组融合蛋白易纯化并具有高活性。
CONCLUSION: The mouse endostatin recombinant fusion protein expressed in E. coli by using thioredoxin fusion expression system is easy to be purified and possesses high activity.
目的：合成胆汁三烯结合蛋白(BBP)基因并在大肠杆菌中表达，获得重组BBP纯化制品。
Objective: To synthesize bilin binding protein (BBP) gene sequence, express BBP efficiently in Escherichia coli and purify the recombinant protein.
重组人脑源性神经营养因子（BDNF）在大肠杆菌中表达，以冻干形式提供。反相高效液相色谱法和SDS-PAGE测定其纯度大于96％。
Recombinant human BDNF was expressed in E. coli and is supplied in a lyophilized form. A greater than 96% purity was determined by reverse phase-HPLC and SDS-PAGE.
目的：构建弓形虫棒状体蛋白（ROP2 ）基因重组质粒并在大肠杆菌中表达，用于筛选弓形虫新的诊断抗原和疫苗分子。
Objective:To construct a recombinant plasmid containing rhoptry protein 2(ROP2)gene of Toxoplasma gondii and express it in E. coli for selection of diagnostic antigen and vaccine candidate.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒，稳定高效地获得基因工程产品心钠素。
Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
目的：在大肠杆菌中表达具有活性的重组人白细胞介素- 29 (IL - 29)。
AIM: To express recombinant human interleukin-29 (IL-29) with biological activity in E. coli.
目的：在大肠杆菌中表达重组单纯疱疹病毒2型(HSV - 2)抗原。
Objective: To clone and express recombinant HSV-2 antigen in E. coli.
研究发现，重组的碱性蛋白酶在进入大肠杆菌周质空间时可能存在前肽自动脱落的现象。
The study also discovered that while the recombinant alkaline protease was secreted into the periplasmic space of e.
利用鹦鹉热衣原体重组主要外膜蛋白（MOMP）在大肠杆菌中的表达产物，纯化后与白油佐剂乳化成疫苗。
The purified recombinant major outer-membrane protein (MOMP) of Chlamydia psittaci(Cps)expressed in E. coli was mixed with oil adjuvant and manufactured as MOMP-vaccine.
对重组肠激酶在大肠杆菌中的高密度发酵表达条件、纯化方法及其生物活性测定进行了研究。
The optional high cell-density fermentation conditions, purification methods and biological activity of the recombinant enterokinase in E.
以纯化的重组F41菌毛蛋白作为检测抗原，建立了检测产肠毒素大肠杆菌F41菌毛抗体的间接ELISA方法。
An indirect enzyme-linked immunosorbent assay (ELISA) was developed based on a purified recombinant F41 pili protein of enterotoxigenic Escherichia coli (ETEC).
目的：在大肠杆菌中表达具有活性的重组人白细胞介素- 29 (IL - 29)。
AIM: To express recombinant human interleukin-29 (IL-29) with biological activity in e.
对大肠杆菌表达的重组人胰岛素原包涵体蛋白的变性复性条件进行了优化。
Optimization of the renaturation conditions for recombinant human proinsulin inclusion bodies expressed in E.
目的构建大肠杆菌双杂交系统的诱饵重组质粒。
Objective To construct a bait plasmid in bacterial two-hybrid system.
目的：探索重组人胎盘抗凝蛋白变体大肠杆菌表达菌株的最适表达条件。
Objective the purpose of this study is to optimize the different parameters in the expression of recombinant human placental anticoagulant protein derivative expressing e.
重组蛋白在大肠杆菌中表达多为无活性的包涵体形式，须经洗涤、溶解、复性后才能得到生物活性蛋白。
Expression of recombinant proteins in Escherichia coli often results inclusion bodies, an aggregation form of inactive proteins.
结论利用大肠杆菌表达的HEV重组抗原建立了双抗原夹心法elisa，并可同时用于不同动物的抗hev抗体检测。
Conclusion a double antigen sandwich ELISA have been established using recombinant antigens expressed in E. coli., and can be used to detect anti HEV in different kinds of animals.
将含突变基因的重组质粒转化大肠杆菌菌株bl2 1 (DE3)，经IPTG诱导表达获得的目的蛋白质均以可溶形式存在。
The recombinant plasmids containing mutant genes were transformed into the Escherichia coli strain BL21 (DE3), and the expressed proteins were found to be water soluble after the induction of IPTG.
结论：成功在大肠杆菌中获得高效表达CFP32蛋白，血清抗体检测表明重组的CFP32蛋白有希望成为结核病血清学诊断的有效候选蛋白之一。
Conclusions: The recombinant CFP32 proteins was successfully expressed in E. coli and purified, Elisa analysis showed recombinant CFP32 may be a candidate antigen for TB serodiagnosis.
重组质粒经酶切和测序证实正确，然后转化大肠杆菌BL21。
The recombinant plasmid was confirmed by digestion and sequence, then transformed into E. coli BL21.
拟获得在大肠杆菌中能高效可溶表达的转铁蛋白受体单链抗体与链亲和素（SA）的重组融合蛋白。
To obtain the anti-transferrin receptor scFv and streptavidin(SA) recombinant fusion proteins which were high-efficiently and solubly expressed in E.
本发明提供了一种在大肠杆菌中生产可溶性的、有生物活性的重组人血管抑素的工艺和分离纯化方法。
The expressed and purified recombined human blood vessel chalone can suppress the amplification of endothelial cell specifically in dosage dependence.
结果构建了具有正确基因序列的CFP10重组表达质粒，重组蛋白在大肠杆菌BL21（DE3）中以包涵体形式表达。
The recombinant CFP10 protein was expressed in inclusion body in E. coli BL21(DE3), and the target gene had been cloned into host bacterium.
将重组质粒转化至大肠杆菌宿主中，对目的蛋白进行诱导表达，并对诱导表达条件如温度、IPTG浓度等进行了优化。
The expression condition of plasmid pET-tmcC has been explored, but at all the testing temperature, IPTG concentration and different hosts protein expression was not found.
将重组质粒转化至大肠杆菌宿主中，对目的蛋白进行诱导表达，并对诱导表达条件如温度、IPTG浓度等进行了优化。
The expression condition of plasmid pET-tmcC has been explored, but at all the testing temperature, IPTG concentration and different hosts protein expression was not found.
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