结论：重叠延伸pcr是一种获得重构基因的简捷方法。
Conclusion Splicing overlap extension by PCR is a convenient method to obtain recombinant gene.
重叠延伸PCR技术成功的关键是重叠互补引物的设计。
The overlap primers design is the key factor for the successful accomplishment of SOE PCR.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
方法用PCR方法扩增il_3信号肽序列和人内皮抑素基因，然后采用重叠延伸pcr拼接的方法将两者拼接和扩增。
METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR.
对常规重叠延伸PCR技术进行了改进，改进后的方法称之为一步法重叠延伸pcr，减少了进型PCR反应的次数，提高了效率。
The common overlap extension PCR was improved and the new methods named as one-step overlap extension PCR, which can reduce the PCR times and improve the efficiency.
对常规重叠延伸PCR技术进行了改进，改进后的方法称之为一步法重叠延伸pcr，减少了进型PCR反应的次数，提高了效率。
The common overlap extension PCR was improved and the new methods named as one-step overlap extension PCR, which can reduce the PCR times and improve the efficiency.
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