方法:取瘢痕疙瘩和正常皮肤,采用组织块培养法进行成纤维细胞体外培养。
METHODS: Keloid and norm al skin tissue were collected for the fibroblasts in vitro using tissue-block cultivation.
方法:取5-10周流产人胚胎生殖腺嵴,采用组织块培养法培养人EG细胞。
Methods: Through tissue culture, the human EG cells were obtained by taking the gonadal ridges from human embryos aged 5~10 weeks.
方法采用组织块培养法培养羊膜细胞,酶消化法对细胞进行传代,应用免疫细胞化学的方法对细胞进行鉴定。
Methods Amnion cells from human were cultured with tissue piece method, passaged by trypsin digestion and identified with immunocytochemistry.
方法血管平滑肌细胞采用组织贴块法培养。
Methods Vascular smooth muscle cells were cultured by explant method.
方法:分别采用组织块法、酶消化法、组织块酶消化法进行人牙髓细胞原代培养。
METHOD: Human pulp cells were primary cultured with tissue block method, enzymolytic method, and tissue block enzymolytic method separately.
采用组织块贴壁培养法,成功地构建北京鸡成纤维靶细胞系。
Using the organization to adherent culture, we succeeded in constructing Beijing fatty chicken fibroblast specific target cell.
方法采用低钙培养法对人角膜缘基底层细胞组织块进行培养。
Methods Limbal button with basal cells was cultured in low calcium medium.
方法:采用组织块贴壁法培养大鼠主动脉平滑肌细胞。
Methods: VSMCs were cultured by tissue explants adherent method.
分别采用酶消化法和组织块培养法,建立了猪小肠黏膜上皮细胞原代培养的方法。
To establish the method on the primary culture of porcine intestinal epithelial cell, different digestion protocols and explant culture were used to identify the best method.
采用组织贴块法分离培养兔平滑肌细胞。
Rabbit smooth muscle cells were isolated and cultured using tissue explant method.
方法采用显微解剖法分离出耳蜗血管纹,组织块培养法进行体外培养。
Methods Cochlea stria was separated by micrergy from guinea pigs and the stria tissue nubbles were cultured in vitro.
分别采用酶消化法和组织块培养法,建立了猪小肠黏膜上皮细胞原代培养的方法。
The method of explant culture was used successfully in this study to establish neonate goat intestinal epithelial cell(IEC)line.
分别采用酶消化法和组织块培养法,建立了猪小肠黏膜上皮细胞原代培养的方法。
The method of explant culture was used successfully in this study to establish neonate goat intestinal epithelial cell(IEC)line.
应用推荐