并把分型结果与采用聚合酶链反应-序列特异性引物(PCR-SSP)获得的结果进行比较。
The results of genotyping were compared with those obtained with the polymerase chain reaction method using sequence specific primers ( PCR-SSP).
方法:采用序列特异性引物聚合酶链反应的方法对汉族72例uc患者和314例正常对照者HLA - DRB1基因分型。
Methods: By using polymerase chain reaction-sequence specific primers, frequency of HLADRB1 alleles in 72 patients with UC and 314 healthy controls were detected.
TTV的基因分型从已报道的TTVDNA部分基因序列来看,TTV DNA具有高度变异性,采用聚合酶链反应(PCR)检测血清ttv DNA是诊断ttv感染的主要手段。
Genotyping of TTV: according to reported part gene order of TTV DNA, it has high variability. To detect TTV DNA in blood serum by PCR is the maim means to diagnosis the TTV infection.
采用误差分析方法导出增量型自动厚度控制(agc)模型设定序列的差分方程。
A differential equation of giving series of incremental AGC (Automatic Gauge Control) model has been derived by the error analysis method.
然后分别提取84例拟进行造血干细胞移植病人及家系成员的外周血d NA,采用RSCA和序列特异性引物体外基因扩增(PCRssp)法平行对照对HLA A基因位点进行分型。
DNA samples of related hematopoietic stem cell transplant donor-recipients were extracted from peripheral blood cells. HLA-A loci were typed both by RSCA and PCR-SSP.
然后分别提取84例拟进行造血干细胞移植病人及家系成员的外周血d NA,采用RSCA和序列特异性引物体外基因扩增(PCRssp)法平行对照对HLA A基因位点进行分型。
DNA samples of related hematopoietic stem cell transplant donor-recipients were extracted from peripheral blood cells. HLA-A loci were typed both by RSCA and PCR-SSP.
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