实验采用原位杂交，NADPH -NBT组织化学，RNA斑点印迹及蛋白质斑点印迹技术。
The experiment was performed by using in situ hybridization, NADPH NBT histochemistry, RNA dot blot and protein dot blot techniques.
方法采用免疫组化及原位杂交技术。
Methods Immunohistochemistry and in situ hybridization technique were adopted.
方法：取试管婴儿助孕技术后未能受精成功的卵细胞，采用多色荧光原位杂交方法检测卵细胞13，16 ，18，2 1和2 2号染色体的情况。
Methods:Multicolor fluorescence in situ hybridization (M-FISH) was applied to check the chromosome status in oocytes for chromome 13,16,18,21 and 22.
方法：取试管婴儿助孕技术后未能受精成功的卵细胞，采用多色荧光原位杂交方法检测卵细胞13，16 ，18，2 1和2 2号染色体的情况。
Methods:Multicolor fluorescence in situ hybridization (M-FISH) was applied to check the chromosome status in oocytes for chromome 13,16,18,21 and 22.
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