方法：采用免疫组织化学方法(SABC法)。
Methods: The immunohistochemical method (SABC method) was applied.
②采用免疫组织化学方法检测各组肾小球中HPA的表达。
The expression of HPA was determined by immunohistochemistry in glomerular in each group.
采用免疫组织化学方法对肿瘤内13种基因的表达进行检测。
The expressions of 13 genes in melanoma were detected by means of immunohistochemistry staining.
方法采用免疫组织化学方法评价肾脏局部ras组分的表达。
Methods Expressions of intrarenal RAS components were assessed by immunohistochemistry staining (IHCS).
方法 采用免疫组织化学方法对人胎大脑额叶NOS阳性神经元的发育进行观察。
Methods The positive expression of the NOS-positive neurons in the frontal lobe of cerebrum of human fetus was observed by immunohistochemistry.
方法 采用免疫组织化学方法研究皮肤血管炎皮损部和非皮损部细胞粘附分子的表达。
Methods Immunohistochemical technique was used to study the expression of cell adhesion molecules in the lesional and non lesional cutaneous vasculitis.
采集olk病损局部黏膜组织标本，采用免疫组织化学方法检测黏膜上皮细胞OPN表达。
Mucosal epithelium tissues of OLK local lesion were collected and examined for the expression of OPN by immunohistochemical method.
方法对36例视网膜母细胞瘤标本采用免疫组织化学方法观察P 16和P 53表达水平。
Methods Immunohistochemical staining was used to determine the expression of P16 and P53 in 36 cases of retinoblastoma.
方法采用免疫组织化学方法及光镜观察成年大鼠肠道各段IEL与LPL的形态和分布特征。
Methods Used immunohistochemistry methods and electron microscope to observe the shape and the distribution of IEL and LPL which come from the mature rat.
方法采用免疫组织化学方法，检测55例卵巢上皮癌术后癌组织石蜡切片中COX-2蛋白的表达；
Methods Expression of COX-2 in 55 cases of epithelial ovarian carcinoma was detected with immunohistochemical method.
方法采用免疫组织化学方法检测4 7例星形细胞瘤组织中MMP2和TIMP 2的表达情况。
Methods The expressions of MMP 2 and TIMP 2 in 47 cases of astrocytoma were detected by immunohistochemical method.
方法：采用免疫组织化学方法及电镜法，观察成年大鼠肠道各段IEL与LPL的形态和分布特征。
Methods:Immunohistochemistry and electron microscope were used to observe the shape and the distribution of IEL and LPL which come from mature rat.
方法：采用免疫组织化学方法检测48例oscc和26例正常口腔黏膜组织中FHIT的表达情况。
Methods Immunohistochemical method was used to detect the expressions of FHIT in 48 cases of OSCC and 26 normal oral mucosa.
方法：采用免疫组织化学方法和图像分析技术观察adam28在小鼠牙胚发育各期的表达分布及差异。
Methods: Immunohistochemistry and image analysis technique were used to observe the expressions of ADAM28 at mouse tooth germ development stages.
采用免疫组织化学方法检测66例鼻息肉患者血清标本和22例正常人血清标本中il - 3的水平。
Methods: Using immunohistochemical methods detect the expressions of IL-3 in serum of 66 patients with nasal polyp and 22 normal people.
方法 采用免疫组织化学方法观察层粘连蛋白和纤维粘连蛋白在正常人胚早期绒毛膜和蜕膜组织中的分布。
Methods Immunohistochmical method was used to observe the distribution of LN and FN in normal chorion and decidual tissue during early pregnancy.
方法采用免疫组织化学方法，检测42例喉癌组织中vegf与MMP - 9表达及肿瘤微血管计数(mvc)。
Methods VEGF, MMP-9 and microvessel count (MVC) were detected by immunohistochemical method in 42 laryngeal carcinoma specimens.
方法：采用免疫组织化学方法检测57例舌癌患者手术标本和10例正常对照组标本的ETS - 1基因的蛋白表达情况。
Methods: Immunohistochemical method was used to detect the ETS-1 protein expression in 57 specimens of tongue carcinoma tissue and 10 specimens of normal tongue tissue (controls).
方法采用免疫组织化学方法检测17例正常口腔黏膜、60例口腔白斑，30例口腔鳞癌组织标本中TFAR19蛋白的表达。
Methods:The expression of TFAR19 was observed in 17 cases of oral normal mucosa, 60 of oral leukoplakia and 30 of oral squamous cell carcinoma by immunohistochemical stain.
方法：采用免疫组织化学方法检测40例病理性瘢痕组织、20非病理性瘢痕组织和20例正常皮肤组织中igf - 1r、PTEN、PCNA的表达情况。
Methods: The expression of IGF-1R, PTEN, and PCNA in 40 pathologic scars, 20 non-pathologic scars, and 20 samples of normal skin tissue were detected using immunohistochemical methods.
方法采用免疫组织化学法，对99例早期胃癌手术切除标本进行检测。
Methods Resected early gastric cancer from 99 cases were studied by immunohistochemical method.
骨形态发生蛋白2与骨桥蛋白检测：采用免疫组织化学染色与蛋白印迹方法。
The changes of BMP2 and osteopontin were detected by immunohistochemistry and Western blotting.
方法：采用跳台实验和免疫组织化学abc法。
Method: Springing to platform test and immunohistochemical ABC were employed.
观察移植神经干细胞存活、迁移、分化情况采用免疫组织化学染色方法。
The survival, migration and differentiation of the transplanted neural stem cells were observed with immunohistochemical staining.
方法采用免疫组织化学的方法检测42例胆囊癌组织中VEGF的表达和微血管密度（MVD ）。
Methods The expression of VEGF and microvessel density (MVD) were detected by immunohistochemistry in 42 cases of gallbladder carcinomas.
方法采用免疫组织化学s P技术，对175例NSCLC手术标本进行FAS的检测，分析其与多种临床因素的关系。
Methods FAS was examined by immunohistochemical S-P technique in 175 specimens of NSCLC patients. Multiple clinical factors were analyzed according to their relation with expression of FAS.
方法采用甲苯胺蓝染色和免疫组织化学方法。
Methods Using toluidine blue stains and immunohistochemical ABC methods.
方法：采用原位细胞凋亡、免疫组织化学及其双染色等技术，对70例肝细胞癌（HCC）和10例慢性肝炎的组织标本进行研究。
Methods: Expression of HCV antigens and apoptosis were demonstrated using IHC and in situ cell death detecting in samples from 70 HCC and 10 chronic hepatitis.
方法采用免疫组织化学SABC法检测48例骨肉瘤、15例骨软骨瘤标本中akt、PTEN表达的阳性细胞。
Methods the expression of AKT, PTEN had be detected for the specimens of 48 osteosarcoma and 15 exostosis with immunohistochemistry SABC method.
方法：采用免疫组织化学PAP法。
Methods: The CEA was studied with immunohistochemical techniques (PAP method).
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