依据聚合酶链反应(PCR)和酶联免疫吸附检测(ELISA)的方法目前被应用于主要食源性致病菌的检测。
Polymerase chain reaction-based (PCR-based) and enzyme-linked immunoabsorbent assay (ELISA) methods are now applied in the detection of major food-borne pathogens.
单克隆抗体用于以免疫学为基础的诊断方法，包括酶联免疫吸附测定和放射免疫测定法。
Monoclonal antibodies are used in immunology-based diagnostic methods, including enzyme-linked immunosorbent assays and radioimmunoassays.
方法采用酶联免疫吸附法对40例特发性肺纤维化患者和30例健康人血清基质金属蛋白酶- 9进行测定。
Methods The serum level of MMP-9 in 40 patients with IPF and 30 healthy subjects was measured by ELISA.
方法：制备SARS患者肺组织包被抗原，采用酶联免疫吸附试验(EL IS A)检测SARS患者血清中特异性抗体。
Methods: Specific antibodies were detected with enzyme linked immunosorbent assay (ELISA) using coated lung tissue as antigen and serum of SARS patients as antibodies.
目的：建立酶联免疫吸附法检测细胞表面标志的方法。
Objective: to set up a method to measure 'cell surface sign with ELISA method.
方法采用酶联免疫吸附试验(ELISA)对311例过敏性皮肤病患者和30名健康对照者血清中吸入性及食入性过敏原进行检测。
Methods The allergen of inhalation and edibility in 311 cases with allergic dermatitis and 30 healthy persons were detected by enzyme-linked immunosorbent assay (ELISA).
方法用酶联免疫吸附法测定尿微量蛋白。
Methods Using Enzyme Immunosorbent Assay detected urine micro protein.
方法：用酶联免疫吸附试验(ELISA)对各类人群的血清进行SARS病毒抗体的检测。
Methods the SARS antibody in serum of vary population were detected by the method of ELISA.
方法采用酶联免疫吸附法（ELISA）检测23例结核性胸膜炎及21例癌性胸液患者血清及胸液的E选择素水平。
Method Eselectin of serum and pleural effusion in 23 patients with tuberculous pleurisy and 21 patients with lung cancer involving pleura was detected by ELISA method.
方法：采用酶联免疫吸附试验法（ELISA）对50例大肠癌患者以及31例正常人CD105浓度进行了检测。
Methods: Plasma level of soluble CD105 was detected by ELISA in 50 patients with colorectal cancer and 31 normal subjects.
方法采用酶联免疫吸附法检测30例重症感染新生儿、28例轻症感染新生儿及28例正常健康新生儿血清il - 8水平。
Methods ELISA was used to determine the level of IL-8 in serum in 30 newborns with severe infection, 28 newborns with mild infection and 28 healthy newborns, respectively.
目的建立检测小鼠肉汁中旋毛虫抗体的酶联免疫吸附试验(ELISA)方法。
Objective To establish the ELISA method for detection of anti-Trichinella antibodies in muscle juice of infected mice.
方法用酶联免疫吸附法（ELISA）测定血浆GMP-140水平，并用免疫组织化学染色法，观察正常肾和狼疮肾组织GMP-140表达。
Methods Plasma GMP-140 was measured with ELISA in LN patients and normal control. GMP-140 expression in the kidney was measured by a microwave-based immunohistochemistry.
方法应用酶联免疫吸附试验(ELISA法)，测定44例原发性肺癌、20例肺部良性疾病和20例正常人血清内皮抑素含量。
Methods Serum levels of endostatin were measured by an enzyme-linked immunosorbent assay (ELISA) in 44 patients with lung cancer, 20 patients with benign lung diseases and 20 normal persons.
方法应用酶联免疫吸附实验方法(ELISA)进行诺瓦克样病毒抗原检测。
Methods Enzyme-linked immunosorbent assay (ELISA) was adopted to determine the antigen of Norwalk-like viruses.
方法用ELISA方法(酶联免疫吸附实验)检测11例慢性肾炎患者与10例正常人外周血cd146的表达。
Methods The plasma levels of CD146 were detected by enzyme linked immunosorbent assay (ELISA) in 11 chronic glomerulonephritis patients and 10 normal controls.
方法采用酶联免疫吸附法（ELISA法）测定50例动脉粥样硬化及15例健康人（对照组）血清TNF含量。
Methods The serum TNF were measured by ELISA method in 50 patients with atherosclerosis and 15 health cases is control subjects.
采用酶联免疫吸附试验测定麻疹抗体阳性率及抗体几何平均滴度，用统计学方法进行分析。
The positive rate of measles antibody and geometrical mean titer were determined with ELISA and statistical analysis was made.
方法：采用同一问卷对强制戒毒所233名吸毒者进行调查，并用酶联免疫吸附试验(ELISA)和确认试验(WB)对吸毒者进行HIV初筛和确认。
Methods: the 233 drug users were surveyed using the same questionnaire, HIV antibodies were screened and confirmed using enzyme-linked immunosorbent assay (ELISA) and Western Blot (WB) respectively.
检测方法：采用酶联免疫吸附实验(ELISA)。
Detection method is enzyme linked immunosorbent assay (ELISA).
方法通过酶联免疫吸附法测定vegf在健康人、肿瘤患者血清及恶性渗出液中的含量。
Methods VEGF concentrations were measured using an enzyme-linked immunosorbent assay in the serum from the healthy donors and in the serum and the malignant effusion from the patients.
目的建立使用间接酶联免疫吸附法(ELISA)检测人血浆抗碳酸酐酶iv抗体的方法，阐明血浆抗碳酸酐酶iv抗体滴度与肾病综征合(ns)、慢性肾小球肾炎(CGN)和2 -型糖尿病肾病(2 - DN)三种肾脏病的联系。
Objective to develop a indirect ELISA method for the detection of plasma anti-CA iv antibody and to evaluate the relationship between anti-CA iv antibody with pathogenesis of ns, CGN and 2-dn.
以抗卵黄脂磷蛋白抗血清作为抗体，卵黄蛋白原作为竞争抗原建立了间接竞争法酶联免疫吸附反应(ELISA)方法来检测雄鱼血液中卵黄蛋白原含量。
An indirect competitive enzyme-linked immunosorbent assay (ELISA) for detecting Vtg content in the blood of male Carassius auratus was developed with antiserum of lipovitellin resistance as an.
方法：收集19例严重烧伤患者的血浆，利用酶联免疫吸附(elisa)法测定血浆中il 8和IL 10水平。
Methods: By using ELISA method, plasma IL8 and IL10 levels were determined in 19 patients with burn injury.
方法：运用端粒酶pcr -TRAP酶联免疫吸附试验(ELISA)测定法对30例肺癌患者及25例肺部良性疾病患者(对照组)的痰液进行端粒酶活性检测。
Methods: The sputa of 30 lung cancer patients and 25 benign lung disease patients (control group) were checked for telomerase activity with PCR-TRAP ELISA.
方法酶联免疫吸附测定(ELISA)测定血浆IL - 18，对36例itp患者和正常对照组血浆IL - 18的水平进行分析。
Methods The plasma IL-18 in 36 ITP patients and 36 normal controls were detected by enzyme linked immunosorbent assay (ELISA).
方法采用酶联免疫吸附法对42例脑出血患者起病第1、3、7天血清NSE水平进行了动态测定。
Methods The serum NSE of 42 patients with cerebral hemorrhage was tested with ELISA at 1,3 and 7 days after the hemorrhage happened.
方法采用酶联免疫吸附试验检测86例星形细胞瘤术后患者放射治疗前后血清S100蛋白水平。
Methods Serum S100 protein levels were detected by enzyme-linked immunosorbent assay in 86 astrocytoma patients in the course of radiotherapy.
方法：收集50例哮喘急性发作期患儿及30例健康儿童的静脉血，采用酶联免疫吸附试验法(ELISA)测定血清il - 17水平。
Method: Blood samples were taken from 50 asthma children and 30 healthy children. ELISA was used to measure the serum levels of IL-17 of these samples.
应用建立的胶体金免疫渗滤法检测日本血吸虫病血清抗体，并以快速酶联免疫吸附试验（F-ELISA）方法检测作平行对照。
Serum antibody of schistosomiasis patients was detected by dot immunogold filtration method(DIGFA)in laboratory and field, and F-ELISA was used as control.
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