方法酶消化法培养人汗腺上皮细胞。
Methods Sweat glands epithelial cells were collected by enzymatic digestion.
结论酶消化法适用于卫星细胞的培养。
Conclusion This methods was suitable for collection skeleton satellite cell.
兔泪腺上皮细胞的分离：采用改进酶消化法。
The isolation of rabbit lacrimal gland epithelial cells was adopted by modified enzyme digesting.
方法改良酶消化法。
方法：用胰酶消化法培养兔rpe细胞并传代。
Methods: The rabbit RPE cells were cultured with trypsin enzyme-digesting technique and passaged in vitro.
组织块培养法对于原代细胞的培养要优于酶消化法。
The method of explanted tissue culture is better than the method of enzyme digestion for the primary cells.
结论：酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
Conclusion The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes.
经酶消化法短期培养成功率为83.3%，明显高于组织块培养法。
The successful rate of the enzymatic digestion culture method was 83.3%, which was prior to the tissue pieces culture method significantly.
方法：采用酶消化法传代培养乳sd大鼠颅盖骨细胞并建立缺氧模型。
Methods: the osteoblasts obtained from the calvaria of neonatal SD rats were cultured by method of enzyme digestion in vitro.
方法采用酶消化法及酶辅助机械分离法分别分离IPE和RPE细胞。
Methods IPE and RPE cells were isolated by enzymatic digestion method and enzyme-assisted microdissection method respectively.
方法结合酶消化法和组织块培养法，体外单层培养人退变纤维环原代细胞。
Method:Primary cells of human degenerative AF were obtained through in vitro monolayer culture combined with enzyme digestion method and tissue pieces culture method.
方法：用胰酶消化法获取人胎儿视网膜色素上皮细胞进行原代及传代培养。
Methods: The fetal RPE cells were cultured with trypsin enzyme digesting technique and passaged in vitro.
对舟形藻（海水），酶消化法的检出率高，其余三种方法几乎检测不到硅藻；
For Navicula, a majority of diatoms could be extracted by enzymatic digestion method, while only a few diatoms could be detected by other three methods.
从安全性和环保角度考虑，或当溺液为海水及硅藻密度较小时，应选择酶消化法；
Enzymatic digestion method should been selected if safety and environmental protection were considered, or the drowning fluid was sea water and less diatoms were found in it.
方法：分别采用组织块法、酶消化法、组织块酶消化法进行人牙髓细胞原代培养。
METHOD: Human pulp cells were primary cultured with tissue block method, enzymolytic method, and tissue block enzymolytic method separately.
分别采用酶消化法和组织块培养法，建立了猪小肠黏膜上皮细胞原代培养的方法。
To establish the method on the primary culture of porcine intestinal epithelial cell, different digestion protocols and explant culture were used to identify the best method.
分别采用酶消化法和组织块培养法，建立了猪小肠黏膜上皮细胞原代培养的方法。
The method of explant culture was used successfully in this study to establish neonate goat intestinal epithelial cell(IEC)line.
表明利用胰酶消化法可以分离培养出纯度高，数量较多且培养时间明显缩短的成骨细胞。
Tryptic digestion could isolate and culture Numbers of osteoblasts with a high purity and obviously shorten the culture time.
改良酶消化法和刮取法收集人输卵管上皮细胞，采用溶血法及差速贴壁法纯化上皮细胞。
Epithelial cells were collected with a modified method which included enzymatic method and scraping, and then the collected cells were purified by hemolysis method and differential adhesion method.
以18天鸡胚睾丸为实验材料，经胶原酶和胰蛋白酶两步酶消化法得到生精上皮细胞悬液。
Testes from 18-day-old embryonic chickens were used to isolate seminiferous epithelial cells by sequential two-step enzyme digestion with collagenase and trypsin.
采集42日龄山羊的瘤胃肌肉组织，采用胶原酶消化法进行了山羊瘤胃平滑肌细胞的体外培养。
Ruminal smooth muscle tissue of goats (42 days of age) was collected, and ruminal smooth muscle cells was cultured in vitro by the method of collagenase digestion.
方法：分别用胰蛋白酶消化法及胃蛋白酶-胰蛋白酶联合消化法制备视网膜血管铺片，比较铺片成功率。
METHODS: Retinal vascular preparations were performed by using trypsin digestion and pepsin-trypsin digestion technique respectively.
方法采用组织块培养法培养羊膜细胞，酶消化法对细胞进行传代，应用免疫细胞化学的方法对细胞进行鉴定。
Methods Amnion cells from human were cultured with tissue piece method, passaged by trypsin digestion and identified with immunocytochemistry.
用胰蛋白酶和胶原酶消化法和组织贴块法培养猪肺动脉干内皮细胞和平滑肌细胞。方法简便易行，成功率高。
Endothelial cells and smooth muscle cells of porcine pulmonary trunk were cultured by enzyme-dispersed and substrate-attached explant methods.
酶消化法进行表皮细胞和成纤维细胞培养，绘制表皮细胞原代生长曲线，MTT法测定传代后表皮细胞的增殖。
The Epidermal cell and fibroblasts culture in vitro were conducted by enzyme digestion. Epidermal cell primary culture was described by growth graph and MTT method was used in subculture cell growth.
结果：组织块酶消化法中组织块解离、贴附和细胞游出状况均优于单纯组织块法或单纯酶消化法，成功率较高。
RESULTS: The decollation and coherence of the tissue block and the cell growth rate of the tissue block enzymolytic group are better than that of the tissue block group and enzymolyfic group.
证实用改良的二次酶消化法能够在短时间内获得大量成骨细胞，且所培养的细胞具有典型的成骨细胞形态和功能。
It was concluded that the modified twice-enzyme isolation method was an ideal technique to obtain and culture osteoblasts with typical characteristics.
材料和方法：采用酶辅助的显微分离法分离新生牛眼ipe细胞，酶消化法分离角膜内皮细胞，进行体外细胞培养。
Material and Methods: From bovine eyes IPE cells were isolated with enzyme-assisted microdissection, corneal endothelium cells with enzyme digestion and then were cultured in vitro.
方法：采用胰酶消化法分离小鼠胚胎第8 . 5天的颅神经管，从小鼠颅神经管中游离出来的细胞即为颅神经嵴细胞。
METHODS: cranial neural tubes were dissected from 8.5 day mouse embryo using trypsin digestion and explanted in culture dishes. Cells dissociated from the neural tubes were cranial neural crest cells.
方法：采用胰酶消化法分离小鼠胚胎第8 . 5天的颅神经管，从小鼠颅神经管中游离出来的细胞即为颅神经嵴细胞。
METHODS: cranial neural tubes were dissected from 8.5 day mouse embryo using trypsin digestion and explanted in culture dishes. Cells dissociated from the neural tubes were cranial neural crest cells.
应用推荐