方法用SPA法在载体上固定系列浓度酶标记抗体，绘制抗体浓度-吸光度曲线，寻找SPA与抗体的最佳结合条件。
Methods Used SPA method to immobilize the enzyme conjugated antibody of different concentrations! and drew the antibody-OD line.
酶免疫分析技术的质量依赖于抗原的纯度、抗体的特异性、合适标记酶的选用，其灵敏度取决于标记酶的高度纯化和高转化率。
The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label.
酶免疫检测(EIA)技术，是根据抗原抗体反应具有高度的特异性，将酶标记物的抗体作为标准试剂来鉴定未知的抗原。
Based on high specificity of antigen-antibody reaction, enzyme immunoassay (EIA) identified unknown antigen by antibody labelled with enzyme as standard reagent.
通过固定辣根过氧化酶（HRP）标记CA19-9抗体在纳米金修饰的碳糊电极制备了CA19-9安培免疫传感器。
A novel immunosensor for the determination of carcinoma antigen-19-9(CA19-9) is made by immobilization of horseradish peroxidase(HRP) labeled CA19-9 antibody on colloidal gold carbon paste electrodes.
本发明也公开了用该细胞系制备单克隆抗体、抗体提纯方法及该抗体的辣根过氧化酶标记法。
The invention also discloses preparation of the monoclonal antibody by using the cell line, a method for purifying the antibody and a horseradish peroxidase labeling method for the antibody.
方法采用方阵滴定法，比较4种封闭液、不同稀释度的捕获抗体、检测抗体和辣根过氧化酶标记链菌素对检测结果的影响，确定ELISA法的最佳实验条件。
Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test.
方法采用方阵滴定法，比较4种封闭液、不同稀释度的捕获抗体、检测抗体和辣根过氧化酶标记链菌素对检测结果的影响，确定ELISA法的最佳实验条件。
Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test.
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