番茄红素的提取方法主要包括：有机溶剂提取法、酶反应法、超临界CO2萃取法等。
The extracted methods of lycopene mainly include: organic solvent extraction, enzymatic reaction extraction and supercritical CO2 extraction.
文章综述了番茄红素的提取方法，包括直接粉碎法、有机溶剂浸提法、超临界流体萃取法、制备高压液相色谱及酶反应法等。
In this paper, the ways of lycopene production was reviewed, including extract by chemicals, supercritical fluid extraction, HPLC, enzyme extraction, and so on.
STR体外酶活性分析采用间接荧光法检测色胺在反应体系的消耗。
In vitro, STR enzymatic assay was measured indirectly by fluorimetrically detecting depletion of tryptamine feeding on secologanin in the reaction mixture.
通过聚合链反应或ELISA酶标法测试，共有超过25例人类样本被证实为裂谷热阳性。
More than 25 human samples have proved positive for Rift Valley fever by PCR or ELISA testing.
通过聚合链反应或ELISA酶标法，已发现25例以上的人类样本为裂谷热阳性。
More than 25 human samples have been found positive for RVF by PCR or ELISA.
聚合酶链反应-微孔板杂交法检测输血传播病毒。
DNA detection of tt virus DNA by polymerase chain reaction -microplate hybridization.
采用单链聚合酶链反应(PCR)和测序法检测肿瘤组织中VHL基因的突变情况。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
酶法提取具有反应条件温和和底物特异性好等特点。
Extracted tea protein by enzyme is mild reaction condition and substrate specialization.
有机相中应用酯化反应，酶法催化拆分消旋醇是获得光学活性醇的重要途径。
The main route for obtaining optically active alcohols is resolution of racemic alcohols catalyzed by enzyme in organic solvents by the esterification reaction.
反转录-聚合酶链反应法检测CXCR4的表达，亦得到相似结果。
Similar results were obtained through detecting the expression of CXCR4 with reverse transcription-polymerase chain reaction.
综述了酶法合成胸苷的反应机理，并列举了6个应用实例。
The reaction mechanism of enzymatic synthesis of thymidine was reviewed, and 6 examples of application were given.
在分子蒸馏预处理和单因素试验的基础上，采用响应曲面法建立脂肪酶催化反应生成的脂肪酸甲酯含量的数学模型。
The fatty acid methyl ester content model produced by an enzymatic reaction was presented by a response surface methodology (RSM) on the basis of molecular distillation and single factor experiments.
目的探讨双重聚合酶链反应（DPCR）法检测痰及支气管肺泡灌洗液（BALF）中军团菌DNA在早期诊断军团菌肺炎的意义。
Objective To investigate the value of duplex polymerase chain reaction (DPCR) in early diagnosis of Legionella pneumonia by detecting Legionella DNA in sputum and bronchoalvelar lavage fluid(BALF).
游离脂肪酶催化法以其酶催化剂生产成本较低，反应速率较快的优点，成为生物柴油制备的新的关注方向。
Due to its advantage of low cost and rapid reaction rate, soluble lipase catalysis has been considered as a promising way to prepare bio-diesel.
方法：利用乙醇氧化酶与过氧化物酶偶联，通过优化反应体系及自动生化分析仪分析参数，采用两点速率法对乙醇进行分析。
Methods: to analyze alcohol by two points rate method after optimizing reaction system and the assay parameter of automatic analyzer using alcoholic oxidase coupling peroxydase.
方法利用聚合酶链反应-序列特异引物(PCR -ssp)法，对189例银屑病患者和273例健康人的HLA - DQA1和DQB1等位基因进行检测。
Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls.
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
酚-氯仿法从外周血中提取基因组dna，聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
目的评价聚合酶链反应(PCR)分子灯塔法检测临床标本中结核分枝杆菌(结核菌)的应用价值。
Objective To evaluate the value of PCR molecular beacon assay. In detecting mycobacterium tuberculosis in clinical specimens.
方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
方法提取肿瘤组织总rna，用反转录聚合酶链式反应(RT - pcr)法进行检测。
Methods Total RNAs of tumor tissues were extracted and were detected by RT-PCR.
方法采用聚合酶链反应扩增该家系患者和健康对照个体atp2c1基因的全部外显子，直接测序法进行DNA测序，100例无亲缘关系的正常人作为对照。
Method all exons of ATP2C1 gene were analyzed with polymerase chain reaction and DNA sequencing in all patients of this family and 100 unrelated population-match controls.
用新近建立的三抗体夹心酶联免疫反应法检测标本的ADAMTS13抗原含量。
The activity of ADAMTS13 was measured by residue collagen binding assay, and antigen by a newly developed sandwich ELISA.
目的介绍一种用四氮唑蓝（MTT）为底物的酶反应定量比色分析法。
Objective To introduce a quantitative colorimetric assay based on a tetrazolium salt (MTT).
采用聚合酶链反应(PCR)方法扩增rho基因第1 ~ 5外显子和第1内含子基因片段，用直接dna测序法筛查rho基因突变。
Extron 1-5 of RHO gene was amplified by polymerase chain reaction (PCR), and the mutation of RHO gene was screened by direct DNA sequence measurement.
新的快速检测技术有聚合酶链式反应(PCR)技术、伏安型生物传感器、自动微生物检测系统(ams)、改良MRS培养基和三磷酸腺苷(atp)法等，较传统方法迅速、准确。
The new rapid determination techniques were polyase chain reaction method (PCR), volt-ampere biosensor method, automatic microbe system method (AMS), improvement of MRS culture medium and ATP etc.
聚合酶链反应和病毒分离培养法对50例孕妇（怀51胎）进行评价的阳性预测值分别为92.0%和93.7%。
The positive predictive values (PPV) of the PCR and virus isolation assessments performed in all 50 pregnancies (51 gestational sacs) were 92% and 93.7%, respectively.
聚合酶链反应和病毒分离培养法对50例孕妇（怀51胎）进行评价的阳性预测值分别为92.0%和93.7%。
The positive predictive values (PPV) of the PCR and virus isolation assessments performed in all 50 pregnancies (51 gestational sacs) were 92% and 93.7%, respectively.
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