目的构建以绿色荧光蛋白（GFP）为报告基因的酿酒酵母表达载体。
Objective To construct saccharomyces cerevisiae expression vector with GFP as report gene.
外源淀粉水解酶能在酵母中高效表达并分泌，其主要因素在于：在构建载体时组入强启动子；
The main factors for high efficiency expression and secretion of exogenous amylum hydrolase in S. cerevisiae are as follows: involvement of strong promoter in carrier construction;
结果成功构建了以GFP为报告基因的酿酒酵母载体，并在酵母中得到表达。
Results Saccharomyces cerevisiae expression vector with GFP as report gene was constructed and expressed successfully.
用表达载体yti - 15转化酵母63号株可得到每升约2毫克的人胰岛素原分泌产物。
Secreted proinsulin amounted to about 2 mg per liter was found in the culture medium of yeast strain 63 transformed by plasmid YTI-15.
用表达载体yti - 15转化酵母63号株可得到每升约2毫克的人胰岛素原分泌产物。
Secreted proinsulin amounted to about 2 mg per liter was found in the culture medium of yeast strain 63 transformed by plasmid YTI-15.
应用推荐