利用毕赤酵母表达具有生物学活性的蛇毒类凝血酶安克洛。
Express ancrod, a snake venom thrombin-like enzyme, in methylotrophic yeast Pichia pastoris.
该突变体可在酵母表达系统实现高效表达。
The mutant may be he efficiency expressed in yeast expression system.
毕赤酵母表达系统是目前最为成功的外源蛋白表达系统之一。
The Pichia pastoris is one of the most successful foreign protein expression system until now.
目的：利用毕赤酵母表达具有生物学活性的蛇毒类凝血酶安克洛。
Objective:To express ancrod, a snake venom thrombin-like enzyme, in methylotrophic yeast Pichia pastoris.
目的构建以绿色荧光蛋白（GFP）为报告基因的酿酒酵母表达载体。
Objective To construct saccharomyces cerevisiae expression vector with GFP as report gene.
酵母表达产物具有抑制蛋白酶活性的作用，该基因和其编码蛋白也可以应用于医药和生化领域。
Its yeast expression product has the effect of inhibiting protease activity. The gene and its coded protein may be also used in medicine and biochemistry fields.
酵母表达系统是在酿酒酵母质粒的发现和酵母转化技术的成熟基础上建立起来的真核生物表达系统。
Yeast expression systems are based on the discover of plasmid in Saccharomyces cere- visiae and success of yeast transformation.
目的：利用巴斯德毕赤酵母表达系统获得重组的人层黏连蛋白LG1-3组件蛋白，为进一步研究LG1-3的结构和功能间的关系奠定基础。
Objective: To express the LG1-3 module of human laminin alpha 4 chain in P. pastoris expression system for studying the function of LG1-3 module.
近年来，甲醇酵母作为一种新的蛋白表达系统，已得到越来越广泛的应用。
Methylotrophic yeast had been applied more and more widely in recent years as a new protein expression system.
这些质粒可用于外源基因在酵母中表达的研究。
These plasmids can be used in the study of foreign gene expression in yeast.
之后让两种蛋白都在具有一种报告基因的酵母细胞中表达。
Both proteins are then expressed in yeast cells that have a reporter gene.
结论：通过密码子优化，能显著提高LZP 3基因在酵母细胞中的表达水平。
CONCLUSION: The expression of LZP3 gene in yeast could be successfully improved by using codon optimization.
本文主要在分子生物学水平概括了酵母铁吸收转运机制和植物缺铁胁迫相关基因及其基因表达的研究进展。
The Fe_acquisitionmechanism in yeast, the genes and gene expression under the condition of Fe deficiency stress for plants are summarized in this paper.
目的比较马尔尼菲青霉菌酵母相和霉菌相蛋白质组表达差异。
Objective To compare the difference in protome expression between yeast form and mould form of Penicillium marneffei.
本研究中，酿酒酵母的基因表达数据被用来建立调控网络。
In our research, gene expression data of Saccharomyces cerevisiae is applied to construct regulatory network.
此重组体可在酵母中进一步表达特异性蛋白。
The recombination EgB can be used for the expression of specific protein in yeast.
从多方面分析了该算法的性能，并将该算法应用于酵母细胞周期的芯片表达谱数据聚类。
The new clustering algorithm is analyzed on several aspects and tested on the published yeast cell-cycle microarray data.
第三种绕过氧化还原反应辅助因子的不平衡的方法是利用一种能够在酵母中表达的木糖异构酶来替换还原酶和脱氢酶。
A third approach that bypasses the REDOX imbalance issue is to replace the reductase and dehydrogenase with one enzyme xylose isomerase that can be expressed in yeast.
用大肠杆菌，酵母，昆虫细胞表达抗原。
目的观察高脂肪高能量对脂肪组织酵母染色质沉默因子1基因表达的影响。
Objective to observe the effect of high fat and high energy intake on silent information regulator 1 (Sirtl) expression in fat tissue.
在小鼠和烟草中，这是通过过渡表达聚羟化酶来实现的，类似于酵母和昆虫细胞培养。
In mice and tobacco, this was overcome by over-expression of prolyl hydroxylase, analogous to what has been done in yeast and insect cell culture.
结论：为下一步在毕赤酵母中组成型表达外源蛋白，研究其作用机理和遗传机制奠定了基础。
Conclusion:The GAP promoter can be used to express foreign protein effectively and is the basis for studying the physicochemical properties and genetic mechanism of Gassericin T.
酿酒酵母系统是最重要的外源基因表达系统之一。
Saccharomyces cerevisiae is one of the most important heterologous expression systems.
甲醇酵母系统由于其在表达蛋白方面无可比拟的优越性 ，已越来越得到广泛的应用。
Methylotrophic yeast is applied more and more widely because of its unparalleled superiority in expressing heterologous proteins.
结论：成功克隆SARS冠状病毒核衣壳蛋白基因全长，并在酿酒酵母中诱导表达成功，有助于抗sars分子疫苗的研究。
Conclusion Successful clone of an integrity SARS-CoV nucleocapsid gene and its effective expression in saccharomyces cerevisiae can be beneficial to the research on SARS vaccine.
甲醇营养型酵母和裂殖酵母作为外源基因表达的有效系统，正引起人们广泛研究。
Methlotrophic yeast and fission yeast S. pombe have received attention as a good foreign genes expression system.
采用ELISA夹心法检测人重组IL - 2在酵母菌中表达产物的免疫学活性。
Sandwich ELISA Method for measurement of the activity of recombinant human IL-2 expressed in yeast was established.
外源淀粉水解酶能在酵母中高效表达并分泌，其主要因素在于：在构建载体时组入强启动子；
The main factors for high efficiency expression and secretion of exogenous amylum hydrolase in S. cerevisiae are as follows: involvement of strong promoter in carrier construction;
外源淀粉水解酶能在酵母中高效表达并分泌，其主要因素在于：在构建载体时组入强启动子；
The main factors for high efficiency expression and secretion of exogenous amylum hydrolase in S. cerevisiae are as follows: involvement of strong promoter in carrier construction;
应用推荐