目的建立运用酚-氯仿法结合磁珠法从蝇蛆嗉囊内提取人类DNA的方法，从而提高STR分型检验的灵敏度。
Objective To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping.
酚-氯仿法从外周血中提取基因组dna，聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
方法对临床分离的15株金黄色葡萄球菌，酚氯仿法提取其DNA后行RAPD分型，同时进行噬菌体分型。
METHODS DNA from 15 clinical strains of S. aureus were extracted by phenol-chloroform method and used in the RAPD assay, phage typing were also performed.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA，比较荧光定量pcr测定的重复性和测定值的差异。
Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法：对临床分离的85株金黄色葡萄球菌，用酚氯仿法提取DNA后进行RAPD分型。
Methods: 85 clinical strains of S. aureus were extracted by phenol chloroform method and the RAPD typing was also performed.
方法：应用本层析柱方法从金鱼组织所提取RNA和采用异硫氰酸胍-酚-氯仿一步法提取RNA的比较。
Methods: To compare the method of the extract of RNA from goldfish using laminar analysis column with that of using one-step guanidinium isothiocyanate-alcohol phenyl-chloroform.
方法：应用本层析柱方法从金鱼组织所提取RNA和采用异硫氰酸胍-酚-氯仿一步法提取RNA的比较。
Methods: To compare the method of the extract of RNA from goldfish using laminar analysis column with that of using one-step guanidinium isothiocyanate-alcohol phenyl-chloroform.
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