方法：采用全细胞膜片钳技术，记录钠通道电流。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
目的探讨心肌梗死后右心室肌细胞离子通道电流的变化。
Objective: To study the current change of right ventricular myocyte ion channel after myocardial infarction.
方法在培养的大鼠海马神经元上用膜片钳全细胞记录钾通道电流。
METHODS Voltage gated potassium outward current was recorded using whole cell patch clamp techniques in the cultured rat hippocampal neurons.
目的研究在奥拉西坦作用下大鼠下丘脑神经元钠钾通道电流的变化。
Objective To study the effect of oxiracetam on the current of sodium and potassium channels in rat hypothalamic neurons.
用膜片箝技术记录心肌细胞钙通道电流，首先必须获得单个心肌细胞。
The first step of recording calcium channel current of cardiomyocytes with patch clamp technique is dissociation of myocytes.
目的：研究血竭对大鼠背根神经节细胞电压门控性钠通道电流的影响。
Objective: to investigate the effects of blood resin on voltage gated sodium channel current in freshly isolated rat DRG neurons.
结论氨基酸受体门控性离子通道电流改变在肝性脑病发生中起一定作用。
Conclusions the changes of the amino acid receptor gated ion channel currents play an important role in the pathogenesis of hepatic encephalopathy.
方法在离体培养的A549细胞上，用膜片钳全细胞模式记录氯通道电流。
Methods the chloride channel currents were recorded in cultured A549 cells by using the whole-cell mode of patch clamp techniques.
目的：研究异丙酚对人肠系膜小动脉平滑肌细胞膜外向钾通道电流的影响。
Objective: To investigate the effects of propofol on outward potassium current in smooth muscle cells isolated from human mesenteric arterioles.
探讨氨基酸受体门控性离子通道电流改变在肝性脑病(HE)发生中的作用。
Objective to explore the effects of the changes of the amino acid receptor gated ion channel currents on the pathogenesis of hepatic encephalopathy (he).
方法：通过膜片钳手段，测量未分化的SHSY5Y细胞钙离子通道电流；
Methods: By using patch clamp technique, measure voltage gated calcium currents in undifferentiated SH SY5Y cell line.
方法：用膜片钳实验技术记录胶原酶急性分离的豚鼠心室肌细胞钾通道电流。
Methods The whole cell patch clamp technique was used in isolated ventricular cells of guinea pigs.
目的：建立大鼠心肌细胞分离方法，用于膜片钳实验，并记录L型钙离子通道电流。
Objective: to devise a method to isolate calcium-tolerant cardiomyocytes from SD rats for the experiment of patch clamp, and record the currents of L-type calcium channels.
急性分离单个的豚鼠气管平滑肌细胞，以膜片钳技术的内面向外式记录单通道电流。
Single channel currents were recorded by using the patch clamp technique in inside-out configuration.
目的：观察溶血磷脂酸(LPA)对豚鼠心肌细胞电压依赖性钾离子通道电流的作用。
Objective To observe the effects of lysophosphatidic acid (LPA) on voltage dependent potassium current in myocytes of guinea pig.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
MethodsAfter rat hippocampal or cerebral cortical neurons were isolated by pronase e, the IA currents were recorded by whole-cell patch clamp technique.
结论电流特性和阿米·诺利敏感性特征表明，生精细胞上所记录到的电流是上皮钠通道电流。
Conclusion the characteristics of such currents and sensitive to Amiloride all approved that these currents recorded from mouse spermatogenic cells were produced through epithelial sodium channels.
笔者应用机械分离获得豚鼠单个初级精母细胞，采用全细胞式膜片钳技术记录该细胞离子通道电流。
Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.
结果：血竭剂量依赖地抑制单个DRG细胞电压门控性钠通道电流，高剂量的血竭抑制作用不可逆。
The inhibition effect was irreversible at high dosage. Conclusion: blood resin inhibits the voltage gated sodium channel currents, which may well provide some analgesic effects.
爆发型模式的通道电流不仅有浓度依赖性和电位依赖性，其升放时间常数也随箝制电位变正而增大。
The unitary current of burst mode was not only dependent on Na concentration in the pipettes but also on the test voltage.
结论CACNA1H基因G 773d突变可使其编码通道电流增加，并可能引起神经元兴奋性增加。
Conclusion CACNA1H gene G773D mutation is able to increase calcium current and neuronal excitability.
目的探讨降钙素基因相关肽(CGRP)对正常及模拟缺血缺氧状态下豚鼠心肌细胞钙通道电流的作用。
AIM To study the effect of CGRP on L-type calcium channel current in guinea pig ventricular myocytes under normal and imitating ischemia and hypoxia conditions.
目的探讨蛋白酪氨酸磷酸化在胚胎鼠主动脉平滑肌细胞系a10细胞容积调节性氯通道电流中的调控作用。
AIM To investigate the relationship between protein tyrosine phosphorylation and volume-regulated chloride channel currents in A10 vascular cells.
方法：采用全细胞膜片钳记录法，在新鲜分离的大鼠背根神经节细胞上观察血竭对钠通道电流幅值的影响。
Method: the whole cell patch clamp recording technique was used in this study. Results: blood resin suppressed amplitude of the sodium channel currents in a dose dependent way.
以原代培养的大鼠海马神经元为标本，运用膜片钳技术的细胞吸附式方法记录NMDA通道的单通道电流。
Methods Cell-attached mode of patch clamp technique were employed to record the single channel current of NMDA receptor with the primary-cultured hippocampal neurons from rats as samples.
结果：(1)建立了膜片钳全细胞记录技术平台，获得了高稳定性的正常心室肌细胞全细胞离子通道电流图。
Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.
方法采用急性分离的小鼠心室肌细胞，低渗溶液激活容积敏感性氯通道电流，膜片钳全细胞记录法记录电流。
Methods Isolated mouse cardiac ventricular myocytes were subjected to whole-cell patch-clamp to record the hypotonicity activated chloride channel currents.
结果表明， 在采集电压门控性离子通道电流时，合理地利用P/N漏减会使离子电流记录更为准确可靠。
Our results indicate that voltage-gated ion channel currents recorded by Clampex will be more accurate and reproducible by properly using P/N leak subtraction.
在I型QT延长综合征中，KCNQ1基因发生突变，对介导延迟整流钾通道电流的复极化钾通道进行编码。
In long-QT syndrome type 1, mutations occur in the KCNQ1 gene, which encodes the repolarizing potassium channel mediating the delayed rectifier IKs current.
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