通过基因芯片技术从基因水平上研究移植排斥相关问题是目前的新趋势。
It is a new tendency to use gene chip to research graft rejection at gene level.
目的:通过基因芯片技术研究薯蓣皂苷对乳腺癌细胞基因表达的影响,初步探索药物作用的靶基因。
Objective: to study the effect of dioscin to breast cancer cytogene expression by cDNA microarray technique, and to explore target genes of drug action initially.
通过研究不同溶剂来溶解pcr产物后,点样对杂交信号的影响,使基因芯片的技术环节得到优化。
By researching how spotting affect the hybridizing signal after dissolving PCR products with different impregnant, we hope to optimize the technology of microarray.
本文通过分析基因芯片的图象特征,提出了基于变形模板技术的基因芯片杂交样点自动识别方法。
In this paper, based on the recognized microarray image features, an automatic spot identification method using the deformable template technique is proposed.
通过对大豆、玉米、油菜、棉花等农作物样品的检测,并由常规PCR技术进行验证,证实基因芯片的检测结果准确可靠,并提高了检测效率,因此,可用于农作物转基因背景的鉴定。
The result obtained from the detection was consistent with that from PCR analyses, indicating that the DNA chip method is reliable and effective for routine analysis of GMOs.
通过对大豆、玉米、油菜、棉花等农作物样品的检测,并由常规PCR技术进行验证,证实基因芯片的检测结果准确可靠,并提高了检测效率,因此,可用于农作物转基因背景的鉴定。
The result obtained from the detection was consistent with that from PCR analyses, indicating that the DNA chip method is reliable and effective for routine analysis of GMOs.
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