方法采用逆转录-聚合酶链反应（RT-PCR）方法。
MethodsReverse Transcription-polymerase chain reaction(RT-PCR) was used.
用逆转录-聚合酶链反应（RT-PCR）法进行检测。
The viral RNA was amplified by a reverse transcription polymerase chain reaction (RT-PCR).
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
用逆转录-聚合酶链反应(RT - PCR)方法检测主动外排泵基因CDR1和CDR2的表达水平。
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of active efflux gene CDR1 and CDR2.
方法：采用逆转录-聚合酶链反应(RT - PCR)检测48例胃癌组织及30例正常组织中vegf基因的表达。
Methods: The expression of VEGF gene in 48 samples of human gastric carcinomas and 30 samples of corresponding normal tissue specimens was detected by RT-PCR.
应用一种缓慢但高度准确的试验，检测病毒的基因物质，叫即时逆转录-聚合酶链反应，或是rRT - P CR，全部做了评价。
All had been evaluated using a slower but highly accurate test called real-time reverse transcription-polymerase chain reaction, or rRT-PCR, which checks for the genetic material of the virus.
方法：应用逆转录-聚合酶链反应(RT - pcr)法检测73例急性白血病患者及23例正常人的WT 1及LRP基因的表达。
Methods: Expressions of WT1 and LRP genes were measured in 73 patients with acute leukemia and 23 normal controls by RT-PCR method.
方法：建立DI大鼠模型，应用逆转录-聚合酶链反应(RT - PCR)方法检测DI及逼尿肌稳定(DS)大鼠逼尿肌组织IP3R亚型变化。
Methods: To set up the model of DI rats, the IP3R isoforms expressions of detrusor tissue in DI rats and ds rats were detected by RT-PCR technique.
有各种逆转录酶聚合酶链反应(扩增核糖核酸基因组RT–PCR)检测试验方法，但灵敏度各不相同。
Various reverse transcriptase–polymerase chain reaction (RT–PCR) methods are available but are of variable sensitivity.
方法采用逆转录聚合酶链反应(RT -PCR)检测84例静脉毒瘾者血浆标本。
Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR).
应用逆转录聚合酶链反应(RT - PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。
Semiquantitative analysis of IL-6 in the hippocampus was done at different time and dose level after whole-brain irradiation with reverse-transcription polymerase chain reaction (RT-PCR).
方法：采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本，通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法采用逆转录—聚合酶链反应(RT -PCR)方法检测40例患者的肺癌组织ttf - 1基因的表达。
Methods 40 patients with lung cancer were studied to detect TTF-1 gene by using reverse transcription-polymerase chain reaction assay (RT-PCR).
结合挖掘结果，运用网络结构可视化思想构建心气虚证网络结构图，并运用逆转录聚合酶链反应(RT - PCR)探索心气虚证基因网络结构。
To integrate the results of clustering data mining and based on visual model requirement, network structure diagram of HQDS would be drawn, gene network structure be study with RT-PCR.
肠道病毒B型，人；脑炎，病毒性；逆转录聚合酶链反应。
Enterovirus B, human; Encephalitis, viral; Reverse transcriptase polymerase reaction.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法应用嵌套式逆转录聚合酶链反应技术检测印记基因p57kip2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。
Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
方法：采用逆转录聚合酶链反应(RT - PCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系a549细胞PAR s的表达情况。
METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.
方法采用逆转录聚合酶链反应（RT- PCR）测定大鼠淋巴细胞CGRP m RNA水平。用放射性标记法测定血浆CGRP水平。
Method Expression of CGRP mRNA in lymphocytes was determined by the semi quantity RT PCR and the CGRP levels in plasma were measured by RIA.
采用逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒基因组M片段G1区基因序列并测序。
G1 gene sequence of m fragment from hantavirus genome was amplified by reverse transcription polymerase chain reaction (RT-PCR) and analyzed.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
逆转录聚合酶链反应(RT - pcr)法测定大鼠十二指肠血管活性肠肽受体1 (VIPR - 1)的表达。
The express of vasoactive intestinal peptide-receptor1 (VIPR-1) in rat dodecadactylon was determined by RT-PCR.
方法采用逆转录聚合酶链反应（RT PCR）方法检测5 2例肺癌组织及远癌正常肺组织SEMA3B基因产物的表达水平。
Methods The expression of SEMA3B was detected in 52 human lung cancer tissues and paired 52 non cancer tissues by To explore the relationship between SEMA3B gene expression and onc RT PCR.
方法采用逆转录聚合酶链反应(RT pcr)法。
经实时荧光定量逆转录聚合酶链反应验证了基因芯片准确可靠。
The results of realtime RTPCR proved the accuracy of the gene chip.
经实时荧光定量逆转录聚合酶链反应验证了基因芯片准确可靠。
The results of realtime RTPCR proved the accuracy of the gene chip.
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