HBV通过特有的逆转录反应进行复制。
有各种逆转录酶聚合酶链反应(扩增核糖核酸基因组RT–PCR)检测试验方法，但灵敏度各不相同。
Various reverse transcriptase–polymerase chain reaction (RT–PCR) methods are available but are of variable sensitivity.
采用逆转录聚合酶链式反应(RT - PCR)检测病毒。
Viral detection by reverse transcription polymerase chain reaction (RT-PCR) assay, and.
应用一种缓慢但高度准确的试验，检测病毒的基因物质，叫即时逆转录-聚合酶链反应，或是rRT - P CR，全部做了评价。
All had been evaluated using a slower but highly accurate test called real-time reverse transcription-polymerase chain reaction, or rRT-PCR, which checks for the genetic material of the virus.
由于病毒存在于“宿主”中，不受免疫反应的影响，根除并不容易，即使在经历了十年抗逆转录病毒治疗之后也是如此。
Eradication will not be easy because the virus stays in these 'reservoirs' and is not affected by the immune response, even after 10 years of antiretroviral treatment.
艾滋病的抗逆转录病毒治疗（ART）的副反应影响到生活质量（QOL）和看护的持续性，并影响卫生保健的结果。
Side effects from antiretroviral therapy (ART) for HIV impact quality of life (QOL) and adherence to care, and they influence decisions about health care.
逆转录多聚酶链反应检测间质胶原酶及金属蛋白酶组织抑制因子- 1基因表达水平。
The gene expression levels for interstitial collagenase and tissue inhibitor of metallo-proteinase-1 (TIMP-1) were measured by reverse transcription polymerase chain reaction.
方法采用逆转录聚合酶链反应(RT -PCR)检测84例静脉毒瘾者血浆标本。
Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR).
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
目的评估用逆转录聚合酶链式反应(RT PCR)方法检测胃癌腹腔微转移的临床病理意义。
Objective to evaluate the clinicopathologic significance of the detection of peritoneal micrometastases in gastric cancer by reverse transcriptase-polymerase chain reaction (RT-PCR).
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法采用逆转录—聚合酶链反应(RT -PCR)方法检测40例患者的肺癌组织ttf - 1基因的表达。
Methods 40 patients with lung cancer were studied to detect TTF-1 gene by using reverse transcription-polymerase chain reaction assay (RT-PCR).
用逆转录-聚合酶链反应(RT - PCR)方法检测主动外排泵基因CDR1和CDR2的表达水平。
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of active efflux gene CDR1 and CDR2.
采用半定量逆转录多聚酶链反应(RT - PCR)检测局部内皮素系统ET - 1、ETAR、ETBR及ECE的基因表达。
The gene expression of ET-1, ETAR, ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response (RT-PCR).
方法采用逆转录-聚合酶链反应（RT-PCR）方法。
MethodsReverse Transcription-polymerase chain reaction(RT-PCR) was used.
方法应用嵌套式逆转录聚合酶链反应技术检测印记基因p57kip2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。
Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
肠道病毒B型，人；脑炎，病毒性；逆转录聚合酶链反应。
Enterovirus B, human; Encephalitis, viral; Reverse transcriptase polymerase reaction.
用逆转录-聚合酶链反应（RT-PCR）法进行检测。
The viral RNA was amplified by a reverse transcription polymerase chain reaction (RT-PCR).
采用逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒基因组M片段G1区基因序列并测序。
G1 gene sequence of m fragment from hantavirus genome was amplified by reverse transcription polymerase chain reaction (RT-PCR) and analyzed.
应用逆转录聚合酶链反应(RT - PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。
Semiquantitative analysis of IL-6 in the hippocampus was done at different time and dose level after whole-brain irradiation with reverse-transcription polymerase chain reaction (RT-PCR).
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
方法：采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本，通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
结合挖掘结果，运用网络结构可视化思想构建心气虚证网络结构图，并运用逆转录聚合酶链反应(RT - PCR)探索心气虚证基因网络结构。
To integrate the results of clustering data mining and based on visual model requirement, network structure diagram of HQDS would be drawn, gene network structure be study with RT-PCR.
方法：采用逆转录巢式酶链免疫反应，对41例结直肠癌组织及其配对的癌旁正常组织和25例良性腺瘤的FHIT基因进行检测。
Methods: Matched normal and cancerous tissues from 41 patients with colorectal cancer and 25 patients with colorectal adenoma were examined for FHIT expression using RT-PCR.
方法：采用逆转录-聚合酶链反应(RT - PCR)检测48例胃癌组织及30例正常组织中vegf基因的表达。
Methods: The expression of VEGF gene in 48 samples of human gastric carcinomas and 30 samples of corresponding normal tissue specimens was detected by RT-PCR.
方法应用逆转录酶-多聚酶链反应(RT - PCR)方法，检测了83例食管癌和贲门癌组织及28例患者45枚淋巴结中MRP基因的表达，并与相应癌旁组织进行对照分析。
Methods RT-PCR was applied to study expression of MRP gene in tumor tissues from 83 cases of esophageal and cardiac carcinoma, and 45 lymph nodes from 28 patients.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
目的构建反义及正义波形蛋白重组逆转录病毒载体，研究波形蛋白在反应性胶质化中的功能与作用。
Objective to construct antisense and sense vimentin retrovirus vector for investigating the effect of vimentin on reactive gliosis.
目的构建反义及正义波形蛋白重组逆转录病毒载体，研究波形蛋白在反应性胶质化中的功能与作用。
Objective to construct antisense and sense vimentin retrovirus vector for investigating the effect of vimentin on reactive gliosis.
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