连续传代证明4个融合株的双亲遗传特性是稳定的。
After a series of passages, the hereditary properties of the 4 fusion strains were found to be stable. Micechallenged.
本文探讨了连续传代培养中营养对昆虫病原线虫繁殖力及产量的影响。
The effects of nutrition on fecundity and yield of nematode in continued culture were evaluated.
方法切取肾母细胞瘤患儿肿瘤组织植入裸小鼠右前肢皮下，连续传代。
Methods Fresh human Wilms' tumour specimens were sliced and inoculated hypodermically into the forelimbs of nude mice.
在丰富培养基中连续传代12代后，对酚类化合物的降解性能没有改变。
After 12 generations of serial cultivation in rich medium, the degradation ability of phenolic compounds has not changed.
结果建成细胞系CM 319，经近两年的体外培养，已连续传代百余次。
Results The newly established cell line CM 319 has been maintained in continual cultures for over 100 generations in two years.
病毒连续传代至第7代或第8代以后，其感染率、病毒滴度及多角体产量均有显著下降。
Infection rates, titer, and OB yield declined with increasing serial passages of MsNPV in the cell line, they dropped off rapidly after the 7th or 8th passage.
目的：研究甲肝病毒在细胞内快速连续传代培养病毒增殖情况，探索疫苗生产工艺改进的可能性。
Objective: to study the proliferation of HAV which were cultured rapidly and consecutively in cells, and to investigate the possibility of improving the production process of inactivated HAV vaccine.
同时在连续传代40代后每隔5代提取重组病毒全基因组进行酶切鉴定，发现其酶切位点保持不变。
To evaluate its heredity stability, the recombinant virus genome was extracted and digested by Hindlll every 5 generations, and 40 generations later, its enzyme digestion sites almost keep stable.
结果：小型香猪角质形成细胞呈“铺路石”样生长，可连续传代，实验结束时成功地将角质形成细胞传至第13代。
RESULTS: The keratinocytes of mini fragrant pigs grew like pave road stone, which can be subcultured serially. The keratinocytes were passed to 13 passages till the experiment was completed.
据报道，ES细胞目前已有在体外培养两年，连续传代达450次以上，而胚胎生殖干细胞传代次数最多可达70~80次。
According to reports, ESC culture in vitro was more than two years and attained 450 serial passages. Embryonic germline ESC attained 70~80 passages at most.
将猫的心、脑、舌用盐酸-胃蛋白酶溶液消化处理后接种小白鼠，经连续传代分离弓形虫虫株，用特异PCR方法对所分离的虫株进行了鉴定。
The objective of the present study was to isolate Toxoplasma gondii strains from cats and then to identify these strains by specific PCR assay. The T.
本研究用犊牛睾丸细胞及MDBK细胞从我国内蒙古自治区达拉特旗发病的进口小尾寒羊唇病理痂块中分离到一株病毒，并对其进行连续传代至病变稳定。
From the scab of the sick sheep , one strain of virus was isolated using Bovine Testicle cell and MDBK cell, then, it was not taken cell passaged until the CPE is order .
应用PCR方法检测组织细胞培养物中的病毒增殖和增殖滴度，测定了PCV2病毒收获时间，试验表明PCV2病毒在PK15组织培养可连续传代，增殖病毒。
PCR method was applied to detect the proliferation condition and titer of PCV2 and to determine the harvest time of PCV2. The results showed that PCV2 could proliferate and passage on PK15 cells.
应用PCR方法检测组织细胞培养物中的病毒增殖和增殖滴度，测定了PCV2病毒收获时间，试验表明PCV2病毒在PK15组织培养可连续传代，增殖病毒。
PCR method was applied to detect the proliferation condition and titer of PCV2 and to determine the harvest time of PCV2. The results showed that PCV2 could proliferate and passage on PK15 cells.
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