目的:建立大鼠脑组织线粒体的体外蛋白合成体系并对其合成产物进行电泳分离和分子量鉴定。
Aim: to establish a system of mitochondrial translation in vitro of rat brain and identify its production of protein by molecular weight.
对毛细管电泳分离条件进行优化的操作者会具有启发作用。
And it may be used for the optimizing of capillary electrophoresis separation conditions.
方法:比较以往报道的各种低分子量蛋白的电泳分离方法,对电泳缓冲液系统、分离胶和浓缩胶的浓度、电泳程序等进行了优化。
Methods: Based on several reported SDS-PAGE systems for low molecular weight protein, the buffer systems, concentrations of separating gel and stacking gel, electrophoresis procedures were optimized.
本论文的研究内容是进行基于电驱动的连续试样引入-芯片毛细管电泳分离分析的研究。
The present work is focused on developing electrokinetic continuous sample introduction systems for microfluidic chip-based capillary electrophoresis.
定量后,分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。
After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE.
电泳分离法分离物质,尤指分离蛋白质的方法,以及基于在电场作用下处于胶粒悬浮状态的各个成分的运动速度基础上进行的分子结构分析方法。
A method of separating substances, especially proteins, and analyzing molecular structure based on the rate of movement of each component in a colloidal suspension while under the.
方法:将1 8种游离氨基酸经自由区带电泳分离后,进行高频电导检测。
Methods 18 underivatized amino acids were separated using capillary zone electrophoresis and consequently detected with high frequency conductivity detection.
对随机引物进行筛选,选出两个引物,对样品进行PCR扩增,用电泳分离产物。
Some arbitrary primers were evaluated, and two of them were selected for the research. All of the sample were amplified by PCR system and separated by electrophoresis.
对随机引物进行筛选,选出两个引物,对样品进行PCR扩增,用电泳分离产物。
Some arbitrary primers were evaluated, and two of them were selected for the research. All of the sample were amplified by PCR system and separated by electrophoresis.
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