目的：构建抗腮腺炎病毒抗体轻链基因重组表达载体。
Objective: To construct the recombinant expression vector of genes encoding light chain of antibody against Mumps Viruses.
抗体轻、重链基因的扩增。
目的：获得抗人视网膜母细胞瘤单克隆抗体轻链可变区基因。
Objective: to acquire the variable region gene of light chain of monoclonal antibody against human retinoblastoma.
核苷酸序列分析表明所克隆基因分别为抗体轻、重链可变区基因。
The nucleotide sequence analyses indicated that the cloned genes coded the variable light and heavy chain domains from mouse antibodies respectively.
推导的氨基酸序列具有典型的抗体轻、重链可变区特征。
Two amino acid sequences deduced had typical features of Ig heavy and light chains' variable regions.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
本发明涉及包含抗体替代轻链序列的构建体和文库。
The invention concerns constructs and libraries comprising antibody surrogate light chain sequences.
本发明涉及包含抗体替代轻链序列的构建体和文库。
The invention concerns constructs and libraries comprising antibody surrogate light chain sequences.
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