目的：获得抗人视网膜母细胞瘤单克隆抗体轻链可变区基因。
Objective: to acquire the variable region gene of light chain of monoclonal antibody against human retinoblastoma.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
目的获取系列鼠抗人CD分子单抗的轻链及重链可变区基因并进行克隆和测序。
Objective to obtain the VH and VL variable region genes of murine McAbs to human CD molecule.
结果：克隆的基因序列符合小鼠轻、重链可变区基因的特征；
RESULTS: The VH and VL genes were homologous with the published gene sequences of mouse antibody variable region.
核苷酸序列分析表明所克隆基因分别为抗体轻、重链可变区基因。
The nucleotide sequence analyses indicated that the cloned genes coded the variable light and heavy chain domains from mouse antibodies respectively.
核苷酸序列分析表明所克隆基因分别为抗体轻、重链可变区基因。
The nucleotide sequence analyses indicated that the cloned genes coded the variable light and heavy chain domains from mouse antibodies respectively.
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