植物基因工程研究的关键问题是向植物中转移外源基因，它主要涉及植物受体的选择和基因转移方法的探索。
Gene transfer is the key point in plant gene engineering research, which covers choosing plant accepter and exploring gene transfer approach.
基因治疗的成功有赖于安全有效的基因转移方法和高水平表达的外源基因。
Successful gene therapy relies on safe and efficient gene transfer methods and high level expression of foreign gene.
结论：外源野生型PTEN基因的转染能导致体外培养的高转移性黏液表皮样癌细胞变性、坏死或凋亡。
Conclusion: the exogenous PTEN gene may induce denaturalization, necrosis, and apoptosis of the highly metastatic mucoepidermoid carcinoma cells.
目的探讨大鼠心脏移植过程中，重组腺病毒介导的外源基因转移至供心的可行性及安全性。
Objective to study efficiency and security of the recombinant adenoviral-mediated gene transfer to the donor heart during the heart transplantation.
结论：超声介导微泡破裂法促进外源基因的转移是一种比较安全而有效的基因转染方法。
Conclusion: Ultrasound-mediated albumin microbubble or SonoVue microbubble destruction method is a promising strategy for gene delivery.
本文介绍了外源基因导入植物的各种新技术的基本原理、操作要点及其在基因转移工作中所取得的成就。
The basic principles and the manipulating key of various techniques for transfering foreign genes into plant and the achievements of transgenic techniques in plant had been introduced.
目的探讨外源野生型PTEN抑癌基因对人高转移性黏液表皮样癌细胞系m 3sp2体外黏附、迁移和侵袭特性的影响。
Objective To study effects of the exogenous wild-type PTEN tumor suppressor gene on in vitro adhesion, migration and invasion of the highly metastatic mucoepidermoid carcinoma cell line M3SP2.
将外源的基因导入生物细胞内必须借助一定的技术方法或载体，目前基因转移的方法分为生物学方法、物理方法和化学方法。
The foreign gene into the biological cells must use a certain technology or approach vector, the current methods of gene transfer into biological methods, methods of physical and chemical methods.
结果：外源基因转移后14天表达尚很稳定，治疗基因在受体关节的滑膜衬里部位表达。
Results Assay of joint lavages confirmed the in vivo expression of biologically active hIL1Ra and hIL-10 and gene expression was not lost 14 days after transfer.
结果：外源基因转移后14天表达尚很稳定，治疗基因在受体关节的滑膜衬里部位表达。
Results Assay of joint lavages confirmed the in vivo expression of biologically active hIL1Ra and hIL-10 and gene expression was not lost 14 days after transfer.
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