目的以电穿孔法转染COS7细胞系以得到较高的转染率。
Objective To optimize the transfection rate in COS 7 cell line with electroporation method.
此外，PEI也被认为具有高转染率和使内涵体破裂的能力。
Additionally, PEI is considered to exhibit high transfection efficiency and endosomal disrupting capacity.
目的：考察不同脂质体材料对阳离子脂质体细胞转染率和脂质体膜流动性的影响。
Objective: To investigate the influences of different lipids on cellular delivery and fluidity of liposome membrane of cationic liposome.
目的比较不同方法制备包裹蛋白脂质体的包封率和转染率，评价蛋白转染的可行性。
Objective To compare the rate of envelopment and the transfection effect of the cationic liposome encapsulated human IgG. In order to appraise the feasibility of protein transfection.
结论LPD是一种制备工艺简单、体外稳定性好、转染率高，具有应用潜力的非病毒载体系统。
Conclusion LPD could be prepared easily with small particle sizes and high transfection activities. LPD may be good non-viral vectors for applications in gene delivery.
如果能够提高基因的转染率，那么在对活细胞进行基因转染时，纳米金就可以作为一种费用有用的媒介。
If the transfection efficiency is improved, gold nanoparticles will be very useful as mediator for gene transfection in living cells.
与缺乏FOL受体的A549细胞相比，由此而来的合成物在过量表达FOL受体的KB细胞中有更高的转染率。
The formulated complexes exhibited a considerably higher transfection efficiency against FOL receptor over-expressing KB cells than FOL receptor deficient A549 cells.
检测转染组和未转染组顺铂杀伤率、细胞凋亡和线粒体膜电位的变化。
Using MTT and flow cytometer, we detected the changes of cell mortality rate, apoptosis and mitochondrial membrane potential.
体外转染乳腺癌细胞，常规动物接种，测量肿瘤体积和动物重量，计算肿瘤抑制率。
The transfected cells were inoculated into nude mice, and the tumor growth inhibition rate was calculated.
在HSF1基因沉默条件下，克隆法观察到转染后的细胞其细胞存活率比未转染的空白对照组明显降低。
The condition of HSF1 gene silence, the survival rate of cells which was in RNAi was much lower than control group by cloning.
结论成功构建稳定沉默CXCR4表达的肾癌a 498细胞株，转染后的细胞凋亡率升高，体外侵袭能力降低，为后续研究奠定了基础。
ConclusionWe have successfully established an A498 cell line with CXCR4 stably silenced; the cell line has a higher apoptotic rate and lower invasion ability, which paves a way for future research.
结论成功构建稳定沉默CXCR4表达的肾癌a 498细胞株，转染后的细胞凋亡率升高，体外侵袭能力降低，为后续研究奠定了基础。
ConclusionWe have successfully established an A498 cell line with CXCR4 stably silenced; the cell line has a higher apoptotic rate and lower invasion ability, which paves a way for future research.
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