然后，通过脂质体转染法将其转入NK-92细胞；
Then the recombinant plasmid was transfected into NK-92 cells with Hpofectamine encapsuled.
结论：基因转染法是建立人膀胱癌多药耐药细胞模型的理想方法。
Conclusion: The establishment of MDR cell lines by gene transfection is an ideal method.
目的比较脂质体介导法和裸质粒直接转染法导入CYP2J3基因表达的效率。
Objective To study the expression efficiency of CYP2J3 gene in rat smooth muscle cells (SMCs) by liposome mediated and naked plasmid transfection.
MTT法显示转染组的细胞增殖力低于未转染组和转染空载体组（P<0.05）。
The MTT showed cell proliferation ability was lower in the transfection group than in the non-transfection and mock-vehicle groups (P<0.05).
目的以电穿孔法转染COS7细胞系以得到较高的转染率。
Objective To optimize the transfection rate in COS 7 cell line with electroporation method.
用MTT法分析顺铂对转染前后的8348细胞抑制增殖和诱导凋亡的能力。
Proliferation suppression and apoptosis of 8348 cells pre and post transfection induced by cisplatin were analysed by MTT methods.
MTT法检测加入反义寡核苷酸后对转染的包装细胞生长的影响。
MTT was used to detect the cell growth of the packaging cells.
结论：超声介导微泡破裂法促进外源基因的转移是一种比较安全而有效的基因转染方法。
Conclusion: Ultrasound-mediated albumin microbubble or SonoVue microbubble destruction method is a promising strategy for gene delivery.
流式细胞仪检测转染效率，MTT法检测细胞毒性。
The efficiency of transfection is examined by Flow CytoMeter and the cytotoxicity is evaluated with MTTassay.
并将外源基因alb亚克隆入该调控序列内，转染c 127细胞，经免疫组化法检测其表达情况。
Subclone the exogenous gene ALB into the regulating sequence for transfection of C127 cells.
ELISA法检测转染上清中肝细胞生长因子的表达，MTT法检测转染脂肪细胞的活性。
HGF expression and the bioactivity of transfected adipose cells were evaluated respectively by ELISA and MTT.
用其体外转染地鼠肾细胞BHK-21，经间接免疫荧光法（IFA）检测，证实了JEV-E的瞬时表达。
The transient expression of JEV E in BHK 21 cells was detected by indirect immunofluorescence assay(IFA) with a monoclonal antibody 2H4 against JEV E.
在HSF1基因沉默条件下，克隆法观察到转染后的细胞其细胞存活率比未转染的空白对照组明显降低。
The condition of HSF1 gene silence, the survival rate of cells which was in RNAi was much lower than control group by cloning.
本研究认为鱼精蛋白改性磷酸钙是一种比共沉淀法合成磷酸钙更好的转染载体。
It could be expected that nucleoprotamine modified calcium phosphate could be a better transfect agent as compared to coprecipitated superphosphate.
方法：人血管形成素- 1重组粘粒与DNA末端蛋白复合物混合后以磷酸钙共沉淀法转染2 93细胞。
METHODS: Angiopoietin-1-PAxCAwt cosmid DNA and adenoviral DNA-TPC were cotransfected to 293 cells by the use of calcium phosphate precipitation method.
结论通过脂质体介导法将HBV全基因转染至肝癌细胞，成功建立了HBV复制状态的细胞模型。
Conclusion We have successfully transfected HBV into HepG2 cells via lipofectamine and established HBV replication models in hepatoma cells.
比较了不同的转染方式对靶基因表达的干扰效果，结果证明电击法的效果略优于浸泡法。
We use two different dsRNA deliveries and the result indicated that electroporation was better than soaking.
比较了不同的转染方式对靶基因表达的干扰效果，结果证明电击法的效果略优于浸泡法。
We use two different dsRNA deliveries and the result indicated that electroporation was better than soaking.
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