采用电转染方法将重组体导入HL-60细胞中；
The recombinant plasmid was transfected into the cell line HL-60 by electroporation.
利用基因转染方法使细胞获得不死性，快速建立小鼠胸腺基质细胞（MTSC）系。
For rapid establishment of mouse thymic stromal cell(MTSC) lines, gene transfection was used to immortalize fresh viable cells.
结论：超声介导微泡破裂法促进外源基因的转移是一种比较安全而有效的基因转染方法。
Conclusion: Ultrasound-mediated albumin microbubble or SonoVue microbubble destruction method is a promising strategy for gene delivery.
方法：用磷酸钙共沉淀的方法向血管成纤维细胞转染MKP1的基因；
Methods: MKP 1 gene was transfected into vascular fibroblasts by the classical calcium phosphate coprecipitation technique.
方法：本课题前期阶段已将含谷氨酰胺酶基因C末端反义序列的质粒转染入胃癌细胞并命名为SGC-7901~（GA）。
Methods: The gastric carcinoma cells had been successfully transfected with plasmid containing the C' distal end antisense sequence of glutaminase gene and called SGC-7901GA in previous researches.
目的：探索优化重组质粒转染细胞后的筛选方法，以得到高纯度U 937转染细胞。
Objective to optimize the selective method to obtain the pure recombinant plasmid transfected U937 cells.
方法将携带P16基因的腺病毒体外转染人膀胱癌t 24细胞，观察腺病毒对T24细胞的转染效率以及P 16基因修饰的T24细胞体外生物学特性的改变。
Methods P16 genes were delivered to the T24 cells jn vitro by adenovirus vectors. Gene transfer efficiency and changes of biological specificity of T24 cells were analyzed.
方法细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。
Methods Transient and stable transfection experiments in cells and assays of chloramphenicol acetyltransferase activity and aromatase activity were used.
目的：探讨小鼠心脏移植中，通过在体外保存期间对供心进行灌注，进行重组腺病毒基因转染的方法及效率。
AIM: To establish a method of in vitro donor heart perfusion in murine cardiac transplantation during preservation and apply it in adenovirus mediated gene transfection for donor heart.
用电泳实验和绿色荧光蛋白标记方法研究了它的转染效率。
We used gel retardation assay experiment and green fluorescence protein marker to investigate their transfer efficiency.
方法：人血管形成素- 1重组粘粒与DNA末端蛋白复合物混合后以磷酸钙共沉淀法转染2 93细胞。
METHODS: Angiopoietin-1-PAxCAwt cosmid DNA and adenoviral DNA-TPC were cotransfected to 293 cells by the use of calcium phosphate precipitation method.
方法采用脂质体介导的质粒转染、流式细胞仪分析、逆转录- PCR及高效液相色谱(HPL C)分析等实验技术方法做有关分析研究。
Methods The plasmid transfection mediated by liposomal transfection reagent, HPLC analysis and reverse transcription-PCR analysis were used in this study.
目的比较不同方法制备包裹蛋白脂质体的包封率和转染率，评价蛋白转染的可行性。
Objective To compare the rate of envelopment and the transfection effect of the cationic liposome encapsulated human IgG. In order to appraise the feasibility of protein transfection.
方法：用脂质体转染携带PTTG基因的表达质粒，构建过表达PTTG基因的垂体细胞株，观察细胞形态。
Methods: Transfect plasmid which carries PTTG gene use liposome, constructs the pituitary cell line which high express PTTG gene, observe the cell form.
结论：基因转染法是建立人膀胱癌多药耐药细胞模型的理想方法。
Conclusion: The establishment of MDR cell lines by gene transfection is an ideal method.
这种含有较多DNA和较高N/P比的多层膜为提高原位基因传递体系的转染效率提供了一种新方法。
The film with more DNA and higher N/P ratio might provide a new method to enhance transfection in localized gene delivery system.
分别研究了在亲水性云母表面和疏水性PD MS表面细胞图案化的方法，并且对这些图案化细胞的基因转染效率进行了研究。
We developed new methods to pattern cells on both hydrophilic mica surface and hydrophobic PDMS surface and investigated the gene transfection efficiency on the patterned cells.
方法构建了不同TPR串联簇的截断突变体的表达质粒后，建立了稳定转染的BEL740 2细胞株。
Methods the plasmids which can express the truncated mutants of the TPR tandem clusters at different region of CRLP were constructed, stable transfected BEL7402 cell lines were established.
该免疫毒素质粒的制备方法包括重组质粒的构建、重组质粒转染的工程菌的制备等步骤。
The preparation process of the immunotoxin plasmid includes the construction of the recombinant plasmid, the preparation of the recombinant transinfected engineering bacterium and other steps.
结论采用绿色荧光蛋白为报告基因的真核细胞转染技术中，脂质体法是效率高、安全性大的方法。
ConclusionUsing GFP as a report gene in eukaryotic cell transfection, the lipofectamine method could get a higher transfection efficiency and higher survival rate for cells.
为了提高受体介导的基因转染效率，本文提出一种基于PEI和FOLOPEGPLL共轭物的新型、简便的合成方法。
For efficient receptor-mediated gene transfection, a new and simple formulation method based on using PEI and FOLPEGPLL conjugate was presented.
为了提高受体介导的基因转染效率，本文提出一种基于PEI和FOLOPEGPLL共轭物的新型、简便的合成方法。
For efficient receptor-mediated gene transfection, a new and simple formulation method based on using PEI and FOLPEGPLL conjugate was presented.
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