无明确的逆转录病毒载体质粒的致突变性的证据。
目的探索逆转录病毒载体在基因治疗乙型肝炎中的应用。
Objective To investigate the effectivness of recombined retrovirus vector in gene therapy.
重组人胰岛素基因逆转录病毒载体质粒应用于活体动物是安全的。
The application to rats of recombinant human insulin gene retroviral vector plasmid mutated in three place with physiological regulatory element constructed by us was safe.
结果：逆转录病毒载体能有效地将外源性基因导入血管内皮细胞并稳定表达。
Results: The CGRP gene could be effectively transferred into HUVEC and stably expressed.
结果表明，自我失活型逆转录病毒载体能很好地用于衰老基因转录调控的研究。
The results demonstrate that self-inactivating retrovirus vector can be used for gene transcription regulation research during the aging process.
构建并鉴定携带人NT - 3基因的逆转录病毒载体以及稳定的包装细胞系。
Objective:To construct a recombinant defective retroviral vector carrying hNT3 gene and a stable virus-producing packaging cell lines , and then to identify them.
结论构建了含hpv16e7的逆转录病毒载体，为人关节软骨细胞的基因转染打下了基础。
Conclusion Successfully constructed retroviral vector encoding for HPV16E7 establishes important basis for gene transfection of human articular chondrocytes.
目的构建携带双自杀基因且可诱导敲除SV40T的逆转录病毒载体，优化目前的肝细胞永生化。
Objective To construct a new retroviral vector with double-suicide genes and knockout-inducible SV40T gene, with the aim of optimizing immortalization of hepatocytes.
目的构建反义及正义波形蛋白重组逆转录病毒载体，研究波形蛋白在反应性胶质化中的功能与作用。
Objective to construct antisense and sense vimentin retrovirus vector for investigating the effect of vimentin on reactive gliosis.
腺病毒载体是继逆转录病毒载体后在基因治疗、基因免疫等方面应用开发得较早且较成熟的一种基因载体。
Adenovirus vector is an earlier and maturer genetic carrier in the respect of gene therapy and genetic immunization after retroviral vector.
目前应用的病毒载体主要有逆转录病毒载体、腺病毒载体、腺病毒相关病毒、慢病毒载体、单纯疱疹病毒载体等。
At present, the mainly applied virus vectors are retroviral vector, adenovirus vector adenovirus-associated virus slow virus vector, herpes simplex virus vector and so on.
结论成功构建了PIG - A基因逆转录病毒载体，感染突变型k562细胞后可以提高其CD59的表达。
Conclusion the retrovirus vector containing the PIG-A gene was successfully constructed and the expression of CD59 on the mutant K562 cells could be increased.
方法构建克隆1个含有JAK2基因和二聚化化学诱导物(AP2 0 187)结合位点所组成的逆转录病毒载体。
Methods a retrovirus vector (RV) which contains JAK2 gene and two binding sites for a chemical inducers of dimerization (AP20187) was constructed.
目的构建含小鼠白细胞介素12双亚基及新霉素磷酸转移酶基因多顺反子逆转录病毒载体，并观察其在小鼠肝癌细胞中的表达。
Objective to construct a polycistronic retroviral vector containing both subunits cDNA of murine IL 12 and NeoR gene and investigate their expressions in murine hepatoma cells.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
结果表明：在双基因逆转录病毒载体介导的小鼠骨髓细胞的基因转导中，IRES与内部SV40启动子相比，更能保证双基因的共同表达。
Results showed that IRES was superior to SV40 promoter for guarantee of genes co expression in bicistronic retrovirus mediated gene therapy.
目的构建携带报告基因增强型绿色荧光蛋白（EGFP）的反转录病毒载体，并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。
Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.
结论F CM分析抗原表达是测定逆转录病毒滴度的有效方法，特别适宜于缺乏选择性基因的载体。
Conclusion Retroviral titer assessment by FCM analysis of antigen expression is effective, especially for vectors lacking a selectable marker.
逆转录病毒介导的基因转移技术要过渡到临床应用，主要解决如何使病毒载体具有高滴度而不具有复制活性。
In order to use retroviral-mediated gene transfer technology in clinical application, retroviral vector must be of high titer and free of detectable replication-competent retroviruses.
逆转录病毒是最为常用的载体之一。
结果：成功地构建了重组BMP7逆转录病毒表达载体，并可在骨髓基质干细胞中表达BMP 7。
The viruses were used to infect directly BMSCs and the expression of BMP 7 gene in BMSCs was analyzed by immunohistochemical staining.
逆转录病毒是最常用的基因治疗载体，但对人类细胞的基因导入率较低。
Retrovirus (RV) is the most widely used vector for gene transfer, but its efficiency is very low for human cells.
目的构建人P 58.1基因的逆转录病毒表达载体并探讨该基因在淋巴细胞中的表达。
To construct the retroviral expression vector of Human P58.1 gene and study its expression in lymphocytes.
方法从Z 10株病毒感染的细胞提取总rna，将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定，应用dnastar软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.
从分子生物学和免疫学的角度介绍埃博拉病毒蛋白，着重介绍GP和VP 30蛋白的结构及其在病毒转录过程中的作用机制，并对其在基因载体上的应用作出展望。
In this paper, the structure of Ebola virus GP and VP30 and their functions in transcription are introduced. Its application on gene carrier is also discussed.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
结论构建了含人pd - 1基因重组逆转录病毒的载体，筛选出稳定表达人PD - 1分子的L929细胞株。
Conclusion HumanPD-1 gene has been cloned, recombinant retrovirus vector containing PD-1 gene constructed and L929 transfection cell line expressing PD-1 molecules selected.
目的构建人胰岛素样生长因子- 1的逆转录表达载体，建立逆病毒介导的IGF - 1基因转移系统。
Objective to construct a retroviral mediated expression system of human insulin-like growth factor-1 (IGF-1), and determine whether bone marrow stromal cells (BMSCs) can be infected by retrovirus.
目的构建人胰岛素样生长因子- 1的逆转录表达载体，建立逆病毒介导的IGF - 1基因转移系统。
Objective to construct a retroviral mediated expression system of human insulin-like growth factor-1 (IGF-1), and determine whether bone marrow stromal cells (BMSCs) can be infected by retrovirus.
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