该方法还被证实可用于快速鉴定转基因物种,有望代替现有的复杂的基因检测方法。
The method has also been confirmed for the rapid identification of genetically modified species, is expected to replace the existing complex gene detection method.
目前,正结合进行田间分离纯合和DNA分子鉴定,培育去除选择标记基因的转基因抗虫玉米自交系。
At present, inbred lines of transgenic insect resistant maize with selective marker gene removed are in separating and inbreeding program assisted by DNA marker detection.
通过对大豆、玉米、油菜、棉花等农作物样品的检测,并由常规PCR技术进行验证,证实基因芯片的检测结果准确可靠,并提高了检测效率,因此,可用于农作物转基因背景的鉴定。
The result obtained from the detection was consistent with that from PCR analyses, indicating that the DNA chip method is reliable and effective for routine analysis of GMOs.
目的为建立能稳定表达人葡糖醛酸转移酶UDPGT1A9蛋白的CHL UDPGT1A9转基因细胞系,并鉴定其对药物的葡糖醛酸缀合活性。
AIM To establish a cell line CHL UDP glucuronosyltransferase gene (UDPGT1A9) which will stably express human UDPGT1A9 protein and determine the activity of expressed UDPGT1A9 in drug glucuronidation.
比较过量表达MYBS3的转基因植株和抑制MYBS3表达的转基因植株的转录谱鉴定出了许多位于MYBS3介导的冷信号传导通路中的基因。
Transcription profiling of transgenic rice overexpressing or underexpressing MYBS3 led to the identification of many genes in the MYBS3-mediated cold signaling pathway.
蛋白印迹实验鉴定转基因植株,ELISA方法测定重组蛋白表达水平,集落形成实验测定重组蛋白活性。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
蛋白印迹实验鉴定转基因植株,ELISA方法测定重组蛋白表达水平,集落形成实验测定重组蛋白活性。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
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