在转基因植物中，启动子是影响转基因表达效率的重要因素之一，选择高效率的启动子是高效率表达外源基因的关键。
It highly effect the expression level of transgenic plant. So choose a high expression promoter is the key to make sure the foreign gene highly express in the transgenic plant.
本发明涉及生物技术中的基因工程领域，公开了一种含绿色荧光蛋白基因的植物转基因表达载体，以及其构建方法和应用。
The invention discloses a plant transgene expression carrier containing green fluorescent albumen gene, its method for constructing the same and use in genetic engineering field of biotechnology.
这只转基因鼠小弟名叫荷比-杰。通过使控制其神经元传输的基因过表达，它走迷宫和记忆玩具的能力得到了极大的提升。
The genetically engineered rat, Hobbie-J, over-expresses a gene that regulates neuron communication, greatly enhancing the rat's ability to navigate mazes and remember toys.
目的获取番茄果实特异性e8启动子基因，为实现外源基因在转基因番茄中果实特异性表达做准备。
Objective to obtain the gene encoding tomato fruit-specific E8 promoter therefore to prepare for exogenous gene transcription and expression in transgenic tomato fruit.
用于细胞基因调控研究的RNA干涉技术和基因诱导表达方法，现在移植到了转基因动物中，并获得了理想结果。
RNA interference methods and gene inducible expression techniques to use on study of gene regulations in cells are transferred to making transgenic animals, and the ideal research results are gained.
新的基因转化程序要求转基因为单拷贝，不带有标记基因，并在不同的转化体中表达一致，稳定遗传。
Transgenes should be genes with single copy and without marker gene, and be able to express and stable inheritable in various transgenic plants in a new gene transformation program.
目的探讨针对乙型肝炎病毒（HBV）x、p双靶区反义RNA对乙型肝炎病毒转基因小鼠HBV复制和表达的影响。
Objective To investigate the effect of dual-target antisense RNA of hepatitis B virus (HBV) targeted to X and P region on replication and expression of HBV gene in HBV transgenic mice.
对转基因植株不同发育阶段的组织和花粉进行组织化学分析，发现P 1943只在双核的花粉细胞中可以检测到GUS表达。
Histochemical analyses of different tissues and pollens at different developmental stages of the transgenic plants showed that P1943 could only direct GUS expression in binucleate pollens.
目的为建立能稳定表达人葡糖醛酸转移酶UDPGT1A9蛋白的CHL UDPGT1A9转基因细胞系，并鉴定其对药物的葡糖醛酸缀合活性。
AIM To establish a cell line CHL UDP glucuronosyltransferase gene (UDPGT1A9) which will stably express human UDPGT1A9 protein and determine the activity of expressed UDPGT1A9 in drug glucuronidation.
该启动子可以驱动外源基因在转基因植物中热激诱导表达，可以代替35s启动子应用于植物基因工程的研究和产业化。
The promoter can drive the heat induced expression of foreign gene in transgenic plant and may be used to replace 35s promoter applied in plant genetic engineering research and industrialization.
目前已经成功构建剪接变体的表达载体，并且获得了各变体的转基因线虫，为下一步的实验奠定了基础。
At present, we have been successfully constructed expression vectors and obtained the transgenic C. elegans, and the results lay the foundation for the next experiments.
经蛋白免疫印记法证实，与同窝野生型小鼠相比，转基因小鼠的脑组织和肌肉组织中TRPV1的表达显著升高。
As detected by Western blotting, TRPV1 expressions in the brain and skeletal muscle of the transgenic mice were significantly higher than the wild-type littermates.
结果提示，选育双重杂合子转基因后代小鼠，是一条提高转基因动物外源基因表达水平的有效途径。
These results indicated that the selection of double heterozygotes among offspring may be a useful way to improve the expression of exogenous genes in the transgenic animals.
免疫组织化学等方法分析得到在转基因阳性鼠肾、脾组织中5-LO、FLAP蛋白的表达明显高于正常鼠。
We also found positive transgenic mice in the kidney and spleen tissues of 5-LO, FLAP protein expression was significantly higher than normal mice by immunohistochemistry.
这些技术策略的应用及改进将提高转基因在质体中特异有效表达，实现外源蛋白在质体中高水平积累。
The application and improvement of these systems will greatly enhance the specific and effective expression of the transgenes, and achieve high-level accumulations of foreign proteins in plastids.
这一单元集中于一些基本的方法来搜索、可视化和分析ZFIN数据，包括基因、基因表达、突变、形态突变、转基因学、解剖结构和抗体。
This unit focuses on some of the basic methods to search, visualize, and analyze ZFIN data, including genes, gene expression, mutants, morphants, transgenics, anatomical structures, and antibodies.
然而，肿瘤基因治疗同样面临一个特殊问题即转基因特异性表达于肿瘤部位问题，靶向性基因转导依然是目前基因治疗的一大障碍。
However, gene therapy for cancer is also faced with a specificity problem: the specific targeting of transgene expression to the site of the tumour.
目的建立系统表达肝素结合性表皮生长因子的转基因动物模型，利用转基因动物模型研究HB - EGF与组织纤维化的关系。
Objective to construct heparin-binding epidermal growth factor-like growth factor transgenic mice in order to investigate the role of HB-EGF in the fibrosis of tissues.
近年来，随着转基因动物制备技术的日趋成熟与完善，建立转基因动物模型已经成为研究真核基因表达调控的一种重要手段。
Recently, with the development of transgenic technology, producing transgenic animal model has become an important way to study the expression regulation of eukaryotic genes.
为满足转目的基因的需求，探索一种能够将外源基因转入银杏细胞，并得以稳定表达和遗传的转基因方法以及选择合适的载体。
To meet the demands of gene transfer of an objective gene, a suitable vector should be screened to carry the extraneous objective gene into the callus cell of Ginkgo biloba.
但同细胞核转基因一样，叶绿体转基因也存在抗生素标记的生物安全性和外源基因的过量表达对植物正常生长发育的影响的问题。
But the same as nuclear transformation, plastid transformation also has the problems of bio-safety of antibiotic markers and development obstacles due to the overexpression of foreign gene.
利用转化有QHB启动子驱动GUS报告基因的转基因植株和原位杂交，我们发现QHB在根静止中心的中央细胞内特异表达。
Using transformants carrying the QHB promoter-GUS and in situ hybridization, we found that QHB was specifically expressed in the central cells of a quiescent center (QC) of the root.
同时，在毕赤酵母里表达抗菌肽的研究为开发转基因酵母作为鱼类饵料添加剂的应用研究提供了理论基础。
Moreover, recombinant expression of antimicrobial peptides in yeast provides us an opportunity to explore the recombinant strains as additive feedstuff of fishery.
蛋白印迹实验鉴定转基因植株，ELISA方法测定重组蛋白表达水平，集落形成实验测定重组蛋白活性。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
比较过量表达MYBS3的转基因植株和抑制MYBS3表达的转基因植株的转录谱鉴定出了许多位于MYBS3介导的冷信号传导通路中的基因。
Transcription profiling of transgenic rice overexpressing or underexpressing MYBS3 led to the identification of many genes in the MYBS3-mediated cold signaling pathway.
因此，我们检测了左心室收缩功能障碍并继发肿瘤坏死因子(TNF1.6)过表达的转基因老鼠腺苷水平和选择性ar的表达。
Therefore, we assessed adenosine levels and selective ar expression in transgenic mice with left ventricular systolic dysfunction secondary to overexpression of tumor necrosis factor - (TNF 1.6).
最后，出现了由神经元特异启动子促发的转基因的报道基因表达的增加，同时出现了依赖于胶质特异启动子的基因表达的下降。
Finally, there is an increase in the expression of a reporter transgene driven by a neuronal-specific promoter and a decrease of gene expression using a glial specific promoter in VPA-treated cells.
本文主要综述了受体基因作为报告基因与治疗基因共同转基因并通过放射性核素显像监测表达的方法。
In the paper we review several strategies to monitor the gene therapeutic efficacy by certain receptor gene as reporter gene transferred together with therapeutic ge...
结论：稳定表达人ox40l的乳腺癌转基因细胞在体外能有效地活化t细胞，介导其增殖、分泌细胞因子及抑制T细胞活化诱导的细胞死亡。
CONCLUSION: OX40L-MDA-MB-435 cells could activate t cells in vitro, promote t cell proliferation and cytokine secretion, and suppress t cell activation-induced cell death.
结论：稳定表达人ox40l的乳腺癌转基因细胞在体外能有效地活化t细胞，介导其增殖、分泌细胞因子及抑制T细胞活化诱导的细胞死亡。
CONCLUSION: OX40L-MDA-MB-435 cells could activate t cells in vitro, promote t cell proliferation and cytokine secretion, and suppress t cell activation-induced cell death.
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