建立草莓主栽品种高效、稳定的离体再生体系和遗传转化体系,获得了转基因植株。
An efficiency and stable regeneration system in vitro and genetic transformation system for the commercial strawberry varieties were developed, and transgenic plants were obtained.
虫试结果表明,转基因植株对蚜虫密度有明显的抑制作用,平均能抑制蚜虫密度为达65.65%。
The results of insect bioassay with peach aphid (Myzus persicae) showed that the transformed plants were aphid ? resistant evidenced by a 6 5.65% reduction in aphid population density.
结果表明:转基因植株生根能力强,根、茎、叶各部位内源激素(IAA、GA3)与对照差异明显;
The results indicated that the rooting ability of transgenic plants was enhanced. Endogenous hormones(IAA, GA3)derived from roots, stems and leaves were significantly different from controls.
蛋白印迹实验鉴定转基因植株,ELISA方法测定重组蛋白表达水平,集落形成实验测定重组蛋白活性。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
采用花粉管通道法遗传转化技术,将慈姑蛋白酶抑制剂基因API导入水稻品系“9311”,获得了转基因植株。
Transgenic rice plant was obtained by transferring the Arrowhead Proteinase Inhibitor (API) gene into rice line "9311" by pollen-tube pathway method.
干旱和盐胁迫导致野生型和转基因型植株丙二醛含量升高,但野生型植株的丙二醛含量增加量要远远高于转基因植株;
MDA contents of both transgenic and wild type plants increased under the drought and high-salt stresses, but MDA contents in wild type plants increased much more than that in the transgenic plants.
更重要的是,与野生型相比,转基因植株在长期的渗透胁迫下会积累更高的鲜重,同时在干旱胁迫中能够保留更多的水分。
Importantly, transgenic plants accumulated higher fresh weight under long-term osmotic stress, and also have shown retention of more water than the wild type during drought stress.
对转基因植株不同发育阶段的组织和花粉进行组织化学分析,发现P 1943只在双核的花粉细胞中可以检测到GUS表达。
Histochemical analyses of different tissues and pollens at different developmental stages of the transgenic plants showed that P1943 could only direct GUS expression in binucleate pollens.
利用转化有QHB启动子驱动GUS报告基因的转基因植株和原位杂交,我们发现QHB在根静止中心的中央细胞内特异表达。
Using transformants carrying the QHB promoter-GUS and in situ hybridization, we found that QHB was specifically expressed in the central cells of a quiescent center (QC) of the root.
比较过量表达MYBS3的转基因植株和抑制MYBS3表达的转基因植株的转录谱鉴定出了许多位于MYBS3介导的冷信号传导通路中的基因。
Transcription profiling of transgenic rice overexpressing or underexpressing MYBS3 led to the identification of many genes in the MYBS3-mediated cold signaling pathway.
产生抗除草剂玉米植株的方法,即用可导致除草剂抗性的转基因转移权利要求2的玉米植株。
A method of producing an herbicide resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers herbicide resistance.
采用根癌农杆菌介导法将油菜CBF1基因转入拟南芥,筛选抗性转基因拟南芥植株,进行PCR检测和GUS染色。
CBF1 gene from rape was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method to screen its transgenic plants with resistance and conduct PCR detection and GUS staining.
转基因番茄子叶、茎段、子叶节经愈伤诱导、分化诱导、分化培养及生根培养能获得再生植株。
Cotyledon, stem and node of transgenic tomatoes can obtain regenerate plants by means of inducing recovery, inducing differentiation, culturing of differentiation and culturing of taking root.
通过对转基因黄瓜和非转基因黄瓜田间农艺性状的观察,转基因黄瓜的T1代植株与对照亲本在主要农艺性状上没有差异。
By watching the field agricultural traits of transgenic and non-transgenic cucumber plants, no differences were found between T1transgenic plants and ck.
通过农杆菌介导,采用叶盘法将豌豆凝集素基因转化单倍体烟草,获得了转基因再生植株。
The pea lectin gene was transformed into the haploid tobacco via Agrobacterium mediation using leaf disc method and the regenerated tobacco plants were obtained.
阐述了转基因水稻抗病植株环境安全性评价的必要性,对环境安全性评价内容提出了个人观点。
The necessity of evaluation on the environmental security of transgenic rice plants was addressed. The author gives his personal views about the contents for security evaluation.
因此,研究大麦成熟胚组织培养条件和植株再生频率的影响因素,建立大麦成熟胚高效再生体系对于有效地开展大麦转基因研究具有重要意义。
So it was significant to build an efficient regeneration system for barley mature embryo by studying factors that influence barley mature embryo culture and plant regeneration.
因此,研究大麦成熟胚组织培养条件和植株再生频率的影响因素,建立大麦成熟胚高效再生体系对于有效地开展大麦转基因研究具有重要意义。
So it was significant to build an efficient regeneration system for barley mature embryo by studying factors that influence barley mature embryo culture and plant regeneration.
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