介绍了基因芯片的类型和制作原理。探讨了基因芯片在植物病害检测和转基因检测中存在的问题和应用前景。
Types and technological principles of gene chips were reviewed, and the problems and prospect of gene chips in plant disease testing and genetically modified organisms detecting were also discussed.
在投喂转基因饲料7周以后以及停止投喂饥饿2周以后分离水体中的微生物,提取其DNA,进行转基因检测。
DNA was extracted from microorganisms in the water at 7-week and continued hunger 2-week. Nested PCR was used to detect whether GM soybean was flow in microorganisms from cultured environment.
专家组说没有必要改变贴标签办法,只有食品或原料改变了特性或含有可以检测到的特异dna的时候才需要贴转基因标签。
The panel said it saw no reason to alter the rule that only foods or ingredients that have altered characteristics or contain detectable novel DNA be required to display a GM label.
为了进一步检测转基因大米的抗病毒性能,研究小组准备首先比较大米和由玉竹提炼而来的纯抗病毒蛋白质的抗病毒性能。
To test the effect further, they plan to compare the antiviral powers of the GM rice that has produced the protein with protein isolated directly from Yuzhu.
当食品或原料发生了特性改变或者含有可被检测的特异dna或蛋白质的时候,要求在标签上标注转基因成分。
Only foods or ingredients that have altered characteristics or contain detectable novel DNA or protein be required to declare the presence of genetically modified material on the label.
摘要论述了2005年转基因农产品,细菌、毒素和微生物,农残、兽残及农产品品质与安全三个方面快速检测技术的进展。
The new advances in fast detection techniques of transgenic farm produce bacilli toxin microbe residual pesticide and antibiotic quality and security control of farm produce in2005are put forward.
我们向应用我们发现含有高水平转基因大豆饲料的那些农民提出,他们能否向我们提供他们的牛奶或鸡蛋样品供我们检测是否存在转基因基因或转基因蛋白质。
We asked those farmers whose feeds we had found contained high levels of GM soya, if they would also provide samples of their milk or eggs for testing for the presence of GM DNA or GM protein.
该方法还被证实可用于快速鉴定转基因物种,有望代替现有的复杂的基因检测方法。
The method has also been confirmed for the rapid identification of genetically modified species, is expected to replace the existing complex gene detection method.
采用根癌农杆菌介导法将油菜CBF1基因转入拟南芥,筛选抗性转基因拟南芥植株,进行PCR检测和GUS染色。
CBF1 gene from rape was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method to screen its transgenic plants with resistance and conduct PCR detection and GUS staining.
建立了玉米转基因成分中35s启动子非同源模板的竞争定量pcr检测系统。
The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35s promoter heterologous template for the detection of genetically modified maize was developed in this study.
目的:检测脑源性神经营养因子(BDNF)靶向药物对阿耳茨海默病(AD)转基因鼠的治疗作用。
Objective: to prepare a targeting drug for brain-derived neurotrophic factor (BDNF) and study its therapeutic effect in Alzheimer's disease (AD) transgenic mice.
为了进一步检测转基因大米的抗病毒性能,研究小组准备首先比较大米和由玉竹提炼而来的纯抗病毒蛋白质的抗病毒性能。
To test the effect further, they plan to compare theantiviralpowers of the GM rice that has produced the protein withproteinisolated directly from Yuzhu.
对转基因植株不同发育阶段的组织和花粉进行组织化学分析,发现P 1943只在双核的花粉细胞中可以检测到GUS表达。
Histochemical analyses of different tissues and pollens at different developmental stages of the transgenic plants showed that P1943 could only direct GUS expression in binucleate pollens.
结果表明,本方法灵敏特异、结果准确可靠,可对目前批准的所有抗除草剂转基因产品进行定性检测。
Results indicated that the method found was sensitive, specific and credible, and all genetically modified crops containing the anti-herbicide genes could be analyzed by this method.
结果表明,建立的多重pcr方法能够准确的同时检测出豆粕中的内源基因和转基因成分。
The results showed that the high efficiency PCR system can differentially, conveniently, effectively and coinstantaneously examine the endogenous gene Lectin and the transgenic components.
抗盐能力检测结果表明,在同等盐胁迫条件下转基因株系生长较快,叶绿素含量和生物学产量都显著高于未转基因对照。
Under the same salt stress conditions, transgenic plants grew faster, had significant higher biomass and chlorophyll content compared to non-transgenic plants.
2005年底以来,三个不同的科学研究小组发表的三篇研究报告以及一篇发表的研究报告事实上在动物组织和牛奶中检测到转基因植物的基因。
Since late 2005, however, three published studies by three different scientific teams and one unpublished study have actually detected transgenic plant DNA in animal tissues and milk.
采用转基因酵母检测系统测定11种原二醇类人参皂苷、4种原三醇类人参皂苷、红景天苷、芦丁和异槲皮素的体外雌激素活性。
The in vitro estrogenic activities of 11 protopanaxadiol type saponins, 4 protopanaxatriol type saponins, salidroside, rutin and isoquercitrin were tested by a recombinant yeast.
国内目前还未见文献报道运用胶体金标记技术检测转基因植物。
Our research is to use the colloidal gold label technology to measure the transgenic plant for the first time, domestic and international not to see the document report yet at present.
随着我国转基因农作物的种植和进口数量的大量增加,转基因食品的安全性问题和检测越来越引起重视。
With the increasing in a large amount of planting and importing of the transgenic crops of our country, the security question and measuring of the transgenic food causes attention more and more.
通过对大豆、玉米、油菜、棉花等农作物样品的检测,并由常规PCR技术进行验证,证实基因芯片的检测结果准确可靠,并提高了检测效率,因此,可用于农作物转基因背景的鉴定。
The result obtained from the detection was consistent with that from PCR analyses, indicating that the DNA chip method is reliable and effective for routine analysis of GMOs.
因此,我们检测了左心室收缩功能障碍并继发肿瘤坏死因子(TNF1.6)过表达的转基因老鼠腺苷水平和选择性ar的表达。
Therefore, we assessed adenosine levels and selective ar expression in transgenic mice with left ventricular systolic dysfunction secondary to overexpression of tumor necrosis factor - (TNF 1.6).
实验结果表明,用改良的CTAB法提纯得到的大豆加工产品中的DNA完全可以满足PCR等分子生物学分析,而且定性PCR法能有效检测大豆及其加工产品中转基因成分。
The results showed that the DNA purified by the modified CTAB method was suitable for PCR and other molecular analysis, and the qualitative PCR was an effective way to screen the GMO.
用MTT法和混合共培养实验分别检测转基因细胞在缺氧或不缺氧状态下对GCV的敏感性以及HRE-TK/GCV系统的“旁观者效应”;
The sensitivity of transfected cells to GCV and "bystander effect (BSE)" of HRE-TK/GCV system under normoxia or hypoxia conditions were measured by MTT assay and mixed coculture experiment.
后来,当我们了解到意大利的研究在商店中买的牛奶中检测到转基因基因,我们也进行了一个类似的,但是较小规模的调查。
Subsequently, when we became aware of the Italianresearch which had detected GM DNA in shop-bought milk, we also carried out a similar, but smaller scale survey.
后来,当我们了解到意大利的研究在商店中买的牛奶中检测到转基因基因,我们也进行了一个类似的,但是较小规模的调查。
Subsequently, when we became aware of the Italianresearch which had detected GM DNA in shop-bought milk, we also carried out a similar, but smaller scale survey.
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