以悬浮细胞作为基因枪轰击的靶材料，转化植株再生频率较初级愈伤组织的为高。
The efficiency of regenerated plant from resistant clones in suspension cells was higher than that in primary callus.
建立草莓主栽品种高效、稳定的离体再生体系和遗传转化体系，获得了转基因植株。
An efficiency and stable regeneration system in vitro and genetic transformation system for the commercial strawberry varieties were developed, and transgenic plants were obtained.
建立高效的植株再生体系是遗传转化的重要基础。
The establishment of effective regeneration system is a very important basis of genetic transformation.
通过农杆菌介导，采用叶盘法将豌豆凝集素基因转化单倍体烟草，获得了转基因再生植株。
The pea lectin gene was transformed into the haploid tobacco via Agrobacterium mediation using leaf disc method and the regenerated tobacco plants were obtained.
胚胎发生能力和再生植株转化率是游离小孢子培养技术的关键问题。
Improving microspores embryogenic ability and plantlet regeneration ability from microspore-derived embryos is the key of isolated microspores culture technique.
植株再生体系的建立是进行基因转化的前提。
The establishment of regeneration system for tall fescue is the foundation for its transformation.
利用高压纸电泳法对毛状根和再生植株进行T-DNA转化的检测。
The transformation of Ri T DNA was examined through high voltage paper electrophoresis.
与棉花体细胞胚胎再生途径相比，棉花茎尖分生组织培养植株成苗的时间短，培养方法简单，也不受基因型的限制，适合作为基因转化的受体系统。
In comparison with embryogenesis, it is easy to get plantlets from shoot apical meristems, and it is suitable and convenience to be used as transformation system.
通过基因枪转化法轰击1160块的愈伤组织得到167株再生植株，转化率17.4%。
By particle bombardment method, 167 regenerated plantlets were gained from 1160 calli, and the transformation frequency was 17.4%.
用RT-PCR 技术检测表明，目的基因在转化再生植株中能够正常转录。
Simultaneously, the result of RT-PCR detection confirmed the target gene has been expressed on the level of transcription.
用RT-PCR 技术检测表明，目的基因在转化再生植株中能够正常转录。
Simultaneously, the result of RT-PCR detection confirmed the target gene has been expressed on the level of transcription.
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