方法贴壁法分离培养外周血单核细胞并进行鉴定。
Methods the monocytes were separated by the anchoring technique and identified.
方法:抽取兔髂骨及胫骨的骨髓液,采用密度梯度离心法结合贴壁培养法分离纯化出MSC,并增殖。
Methods: MSC were aspirated from rabbit iliac and tibias crest and purified by gradient centrifuge and adhesive culture methods.
方法:抽取兔髂骨及胫骨的骨髓液,经密度梯度离心法及塑料板贴壁培养法分离纯化出msc,并增殖。
Methods: MSC were harvested from iliac and tibias crest and obtained by using gradient centrifuge and adhesive culture methods.
方法用梯度离心和贴壁培养法分离msc,进行表面分化抗原的鉴定。
Methods Gradient centrifugation and adherent culture were used to get a great amount of NSC and then differentiation antigens were identified.
结论改良全骨髓贴壁法可有效分离培养大鼠的骨髓间充质干细胞,是一种比较理想的分离培养方法。
Conclusion The improved method of whole bone marrow adherent. can successfully isolate and culture BMSCs in rats, which is proved to be an optimal methods for cell preparation and culture.
结论改良全骨髓贴壁法可有效分离培养大鼠的骨髓间充质干细胞,是一种比较理想的分离培养方法。
Conclusion The improved method of whole bone marrow adherent. can successfully isolate and culture BMSCs in rats, which is proved to be an optimal methods for cell preparation and culture.
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