方法贴壁法分离培养外周血单核细胞并进行鉴定。
Methods the monocytes were separated by the anchoring technique and identified.
采用组织块贴壁法获得了纯化的黑麂耳成纤维细胞。
The present study cultured the homogeneous ear fibroblast cell of the endangered black muntjac.
方法：采用组织块贴壁法培养大鼠主动脉平滑肌细胞。
Methods: VSMCs were cultured by tissue explants adherent method.
方法：组织块反复种植纯化法、低含量胰酶快速消化和差速贴壁法。
METHODS: The repeated implantation, rapid trypsinization, and differential adhesion were used.
结论：胰酶消化、差速贴壁法是一种适合的心室成纤维细胞的培养方法。
Conclusion: Trypsin enzymic digestion and differential attachment was suitable for culture of ventricular fibroblast.
以连续贴壁法筛选大鼠骨骼肌成肌细胞，接种于96孔板进行单克隆培养。
Rat skeletal myoblasts were selected with continuous adherence method for monoclonal cultivation by inoculating into a 96-pore plate.
用悬浮法培养4 ~5天，细胞保持完整的形态结构，用贴壁法细胞可培养数周。
The cells can keep its structure and morphology in 4-5 days usings...
改良酶消化法和刮取法收集人输卵管上皮细胞，采用溶血法及差速贴壁法纯化上皮细胞。
Epithelial cells were collected with a modified method which included enzymatic method and scraping, and then the collected cells were purified by hemolysis method and differential adhesion method.
结论改良全骨髓贴壁法可有效分离培养大鼠的骨髓间充质干细胞，是一种比较理想的分离培养方法。
Conclusion The improved method of whole bone marrow adherent. can successfully isolate and culture BMSCs in rats, which is proved to be an optimal methods for cell preparation and culture.
方法：密度梯度离心法分离44例CHF患者和20例正常人PBMC，酶消化和差速贴壁法分离乳鼠心肌细胞。
Meathod: The experiment adopted density gradient centrifuging to separate PBMC of 44 patients with CHF and 20 normal person and enzyme digestion to separate neonatal rat cardiac myocyte.
微载体培养法是一种将贴壁培养和悬浮培养完全融为一体的较为理想的动物细胞大规模培养方法。
The cultivation on microcarriers (MCS) is a perfect method on the large - scale animal cell culture, which is the complete combination of adhesive culture and suspending culture.
微载体培养法是一种将贴壁培养和悬浮培养完全融为一体的较为理想的动物细胞大规模培养方法。
The cultivation on microcarriers (MCs) is a perfect method on the large-scale animal cell culture, which is the complete combination of adhesive culture and suspending culture.
无菌条件下取正常足月剖腹产的脐带近胎儿段，剔除动静脉，取出其中的间质组织，胶原酶消化法原代培养细胞进行培养和纯化获得贴壁细胞。
Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.
鹿茸细胞采用常规细胞DMSO冻存法复苏贴壁率优于程序式冻胚仪冷冻法、乙二醇常规细胞冻存法。
The antler cell adopt cell routine DMSO frozen methods is superior to program frozen methods and ethylene glycol cell routine freeze methods.
方法：抽取兔髂骨及胫骨的骨髓液，采用密度梯度离心法结合贴壁培养法分离纯化出MSC，并增殖。
Methods: MSC were aspirated from rabbit iliac and tibias crest and purified by gradient centrifuge and adhesive culture methods.
方法：抽取兔髂骨及胫骨的骨髓液，经密度梯度离心法及塑料板贴壁培养法分离纯化出msc，并增殖。
Methods: MSC were harvested from iliac and tibias crest and obtained by using gradient centrifuge and adhesive culture methods.
MTT法检测克隆球细胞和贴壁细胞的增殖能力和对化疗药的敏感性，并植入裸鼠皮下比较移植瘤形成能力；
MTT method was used to measure proliferation capacity and sensitivity to chemotherapeutic drugs, and tumorigenicity was measured by transplanted into the nude mice.
采用组织块贴壁培养法，成功地构建北京鸡成纤维靶细胞系。
Using the organization to adherent culture, we succeeded in constructing Beijing fatty chicken fibroblast specific target cell.
方法用梯度离心和贴壁培养法分离msc，进行表面分化抗原的鉴定。
Methods Gradient centrifugation and adherent culture were used to get a great amount of NSC and then differentiation antigens were identified.
方法用梯度离心和贴壁培养法分离msc，进行表面分化抗原的鉴定。
Methods Gradient centrifugation and adherent culture were used to get a great amount of NSC and then differentiation antigens were identified.
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