用酶切和PCR的方法对重组质粒进行鉴定。
Used restrict enzyme and PCR to verify the reconstructed plasmid.
结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45 - 4b基因。
Results: the constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
结果经pcr方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。
Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.
结果酶切鉴定和DNA测序分析显示，TROP2靶向RNA干扰重组质粒构建成功。
Results Enzyme digestion analysis and DNA sequencing showed that TROP2 targeted RNA interference recombinant plasmids were constructed successfully.
并对氨苄筛选的重组入外源基因的质粒通过P CR、酶切和测序鉴定。
Ampicilin - resistant transformants were selected and identified by PCR, Enzyme digestion and DNA sequencing analysis.
重组质粒经酶切和测序证实正确，然后转化大肠杆菌BL21。
The recombinant plasmid was confirmed by digestion and sequence, then transformed into E. coli BL21.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chain termination method.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chain termination method.
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