目的：构建CD 147反义rna表达质粒载体，探索治疗骨肉瘤侵袭和转移的新方法。
AIM: to construct eukaryotic antisense RNA expression vector of CD147 and probe into a new method to treat invasion and transfer of osteosarcoma.
目的：构建基质金属蛋白酶9靶向的RNA干扰质粒载体，观察其对小鼠巨噬细胞基质金属蛋白酶9基因表达的沉默作用。
AIM: to construct plasmid vector of RNA interference specific for matrix metalloproteinases (MMP) -9 and observe the silencing effect on gene expression of MMP-9 in macrophage of mice.
这些载体具有不同的特征，包括质粒、粘粒和酵母人工染色体。
These vectors can have different features (such as the size of DNA-insert they can accommodate) and include plasmids, cosmids, and yeast artificial chromosomes (YACs).
为构建其启动外源基因的质粒表达载体作准备。
It was therefore preparation for constructing an expression plasmid vector to express exogenous genes.
运用载体重组技术进行质粒重组；
Apply the technique of vector recombine to acquire recombinant plasmids.
实验结果表明，以TK基因构建的重组载体质粒可用于外源基因的重组表达研究。
The result showed that the transfer vector plasmid constructed with FPVTK genes can be used to express foreign gene in FPV recombinant.
实验还大量制备了这两种质粒或载体dna，并用聚乙二醇沉淀法进行了纯化。
These plasmids or vectors DNA were also prepared on a large scale and purified by precipitation with polyethylene glycol (PEG).
目的筛选肝再生增强因子重组质粒基因治疗时较优的载体、途径和剂量及三者的最佳组合。
Objective To select an optimal combined set of carrier, introducing route and dose of recombinant plasmid of augmenter of liver regeneration (ALR) for ALR gene therapy.
综述了近年来有关双歧杆菌质粒分离、载体构建、基因组测序以及在基因治疗上的应用等研究进展。
This paper reviews recent relevant bifidobacterium plasmid isolation, vector, genome sequencing and gene therapy in the application of research progress.
目的：构建人血清及糖皮质激素诱导型蛋白激酶（SGK1）超表达载体质粒，为SGK1的体外研究提供有效的工具。
Objective:To construct plasmid with overexpressed human SGK1 to provide a useful tool for its studies in vitro.
无明确的逆转录病毒载体质粒的致突变性的证据。
重组人胰岛素基因逆转录病毒载体质粒应用于活体动物是安全的。
The application to rats of recombinant human insulin gene retroviral vector plasmid mutated in three place with physiological regulatory element constructed by us was safe.
利用肝细胞生长因子促进血管生成的作用，采用质粒作为载体对大鼠和犬的下肢动脉闭塞疾病模型进行治疗，效果良好。
We used the angiogenesis effect of hepatocyte growth factor to treat the PAD and found the growth of new vessels.
利用肝细胞生长因子促进血管生成的作用，采用质粒作为载体对大鼠和犬的下肢动脉闭塞疾病模型进行治疗，效果良好。
We used the angiogenesis effect of hepatocyte growth factor to treat the PAD and found the growth of new vessels.
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